摘要
试验从影响原生质体制备的诸多方面进行了研究，探索榆黄蘑原生质体制备的优化系统。试验时取鲜嫩的菌丝，用ｐＨ值为６０的ＫＣｌ、蔗糖、甘露醇做酶解稳定液，３０℃下水浴酶解，酶解时间为４～５ｈ，此时榆黄蘑原生质体释放量最大，达到１０７个／ｍＬ。
The experiment was researched in many ways which affect protoplast preparation. A superior system of protoplast of Pleurotus citrinopileutus preparation was studied: fresh Fungus threads were hydrolysised by lywallzyme which was dissolved in KCl or annitol or sucrose in different pH conditions.Under the conditions of T=30 and pH =6 and the time of enzyme hydrolysis during 45 h, the burst rate of protoplast was up to max. The rate of releasion isabout 107/mL.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
1999年第2期26-29,共4页
Journal of Jilin Agricultural University
基金
吉林省科委资助
关键词
榆黄蘑
原生质体
分离
再生
食用菌
育种
Pleurotus citrinopileutus
protoplaste
isolation
regeneration
