摘要
目的 探讨缺血预处理脑保护效应，以及神经细胞凋亡与缺血性脑损害的关系。方法 家兔１５只，随机分为３ 组，对照组（Ａ组，ｎ＝ ５），缺血组（Ｂ组，ｎ＝ ５），缺血预处理组（Ｃ组，ｎ＝ ５）。Ａ组只做手术操作，Ｂ组采用二血管夹闭（双侧颈总动脉夹闭，ＭＡＰ＜ ４０ｍ ｍ Ｈｇ）全脑缺血１０ 分钟，Ｃ组在缺血前增加缺血预处理２分钟再灌注３０ 分钟。对比观察缺血后３ 天海马ＣＡ１ 区神经元密度和缺血细胞数，同时使用ＴＵＮＥＬ原位标记法，检测缺血３天后海马区的凋亡细胞。结果 （１）Ｃ组神经元密度与Ａ 组相比差异无显著性，与Ｂ组相比神经元密度显著增加（Ｐ＜ ０．０５）；（２）Ｃ组缺血细胞数较Ｂ组显著降低（Ｐ＜ ０．０１），与Ａ组相比差异无显著性，缺血预处理对海马ＣＡｌ区内、中、外段分别起到８８％ 、８４％ 、７６％ 的保护作用；（３）ＴＵＮＥＬ法显示Ｂ组短暂脑缺血后迟发性神经元死亡中发现神经细胞凋亡，而Ａ组未发现。结论 （１）缺血预处理后３０ 分钟确能对再次缺血发挥脑保护作用；（２）脑缺血可能促进神经细胞凋亡。
Objective To determine the protective effects of ischemic preconditioning (IPC) on the brain ischemic injury,and to investigate the relationship between apoptosis and brain ischemic injury Methods Fifteen rabbits were randomly divided into three groups group A (n=5);for operative control,group B (n=5) global ischemia for 10min by two vessels occlusion model (bilateral common carotid artery occlusion combined with systemic hypotension),and group C (n=5) ischemic preconditioning for 2 min and reperfusion for half an hour before ischemia Ischemic neurons and neuron density of the hippocampal CA1 region were examined and measured 3 days later Apoptoic cells were measured with paraffin sections stained by TUNEL method Results In group C,neuron density was markedly higher than that in group B (P<0 05) In group C,ischemic neuron number was markedly less than that in group B (P<0 01) IPC protective effects for the medial,middle and lateral subsections of the hippocampal CA1 region were 88%,84% and 76% respectively In group B,apoptotic cells stained with TUNEL method were found in delayed neuronal death after transient cerebral ischemia,but not found in group A Conclusion Ischemic preconditioning protects brain from subsequent ischemia insult Apoptosis may be enhanced by cerebral ischemia
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
1999年第6期348-351,共4页
Chinese Journal of Anesthesiology
基金
云南省应用基础研究基金
关键词
缺血预处理
脑缺血
脱噬作用
Ischemic proconditioning Cerebral ischemia Apoptosis
