摘要
目的探讨人胎阑尾内淋巴组织的发生以及T、B细胞的发育。方法经孕妇本人知情同意收集人胎阑尾26例,HE染色观察微细结构,免疫组织化学SABC法和S-P法染色显示T、B细胞,用BioMiaspro图像分析软件进行计数。结果 HE染色:9周胎儿阑尾管壁4层结构尚未分化形成,上皮为复层柱状,间充质内有单个淋巴细胞散在分布;11周上皮内偶见单个淋巴细胞浸润,间充质内淋巴细胞较前略多;13～15周上皮内淋巴细胞浸润明显,初级淋巴小结雏形形成;16～20周管壁内初级淋巴小结及弥散淋巴组织明显;21～28周淋巴小结初级数量增多,尤以28周明显。免疫组化染色:9周间充质内出现少量IgM阳性细胞;11周出现CD3阳性细胞;15周IgM阳性反应细胞聚集开始形成淋巴小结,小结内CD3阳性反应细胞数量少;随胎龄增加,淋巴小结数量增多,IgM阳性反应细胞和CD3阳性反应细胞数量也逐渐增多(P<0.01,P<0.05)。结论人胎阑尾淋巴组织发生的过程中,9周即有B细胞迁入,而T细胞在11周迁入,B细胞的发育早于T细胞。随胎龄增加,T、B细胞数量逐渐增多。
Objective To investigate the histogenesis of lymphoid tissue and the development of T and B lymphocytes in human fetal appendix.Methods Totally 26 samples of human fetal appendix from the fetus at the 9th to 28th gestational week were collected with informed consent signed by the fetus’ parents.The morphology of the tissues was observed by hematoxylin and eosin （HE）staining.T and B lymphocytes were identified by immunohistochemistry using the streptavidin-peroxidase and SABC methods.The immunoreactive positive-cells were counted using BioMiaspro image analysis software.Results HE-staining showed that the 4 layers of the appendix were not formed at gestational week 9.The epithelium of the appendix was stratified columnar epithelium.There were rare and diffused lymphocytes among mesenchymal cells.At week 11,the lymphocytes were appeared in the epithelium.The amounts of lymphocytes were increasing slightly in the mesenchyme with the elapse of gestational time.The lymphocytes were obviously infiltrated into the epithelium from week 13 to 15.The primary nodules were consisted of a group of lymphocytes.From 16 to 20 week,the primary lymphoid nodules and diffuse lymphoid tissue were seen obviously.At gestational week 21 to 28,the number of primary nodules gradually increased with fetal age,reaching the peak at week 28.Immunohistochemistry indicated that there were few IgM-positive cells were observed in the mesenchyme.At gestational week 11,CD3-positive cells were seen.At week 15,the primary lymphoid nodule were appeared which were gathered by many IgM-positive cells and a small quantity of CD3-positive cells.From 17 to 28 gestational weeks,the amounts of the primary lymphoid nodules and the 2 types of positive-cells were increased gradually with fetal age （P0.01,P0.05）.Conclusion B cells present at the gestational week 9 and prior to T cells by 2 weeks in the appendix.The number of 2 types of positive cells are gradually increased with fetal age.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2010年第16期1766-1769,共4页
Journal of Third Military Medical University
基金
贵州省教育厅自然科学研究项目(黔教科2002116)~~
关键词
人胎
阑尾
B细胞
T细胞
human fetus
appendix
T lymphocytes
B lymphocytes
