期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

嘌呤信号转导研究的新方法 被引量：4

The new methods of purinergic signalling research
暂未订购
导出
摘要 ATP(adenosine 5’-triphophate)是一种嘌呤类物质,参与神经传递和神经调节。随着嘌呤、嘧啶受体亚家族研究的深入,ATP及其受体可能是多种疾病防治的潜在药物靶点。体外研究应用共振能量转移技术,指数级富集的配体系统进化技术及在体小干扰RNA技术可拓宽嘌呤信号转导研究的视野,也将推动其在疾病防治及药物筛选方面的研究进展。 ATP（adenosine 5′-triphophate）is a kind of purine involved in neural transmission and regulation.With the in-depth studying about purine and pyrimidine receptor subfamilies,ATP and its receptor will become a variety of potential drug targets of disease control.The application of resonance energy transfer technology,systematic evolution of ligands by exponential enrichment in vitro and small interfering RNA technology in vivo not only expand the perspective of purinergic signaling research,but also promote their progress in disease prevention and drug screening.
作者 张君 李欣 刘晗 梁尚栋
机构地区 南昌大学基础医学院生理学教研室
出处 《中国药理学通报》 CAS CSCD 北大核心 2010年第7期858-861,共4页 Chinese Pharmacological Bulletin
基金 国家自然科学基金资助项目(No30860086 30860333 30660048) 教育部高校博士点专项科研基金资助课题(No20070403007) 江西省自然科学基金资助课题(No0640042 2007GZY1002 2008GZY0029) 江西省教育厅科研基金资助课题(No2007-60 GJJ08049)
关键词 嘌呤信号转导 荧光共振能量转移 生物发光共振能量转移 BRET-FRET组合法 指数级富集的配体系统进化技术 在体小干扰RNA技术 purinergic signaling FRET BRET SRET SELEX siRNA
分类号 R-05 [医药卫生] R392.11 [医药卫生—免疫学]
  • 相关文献

参考文献25

  • 1Burnstock G.Purinergic signalling:past,present and future[J].Braz J Med Biol Res,2009,42(1):3-8.
  • 2Abbracchio M P,Burnstock G,Verkhratsky A,et al.Purinergic signalling in the nervous system:an overview[J].Trends Neurosci,2009,32(1):19-29.
  • 3Gourine A V,Wood J D,Burnstock G.Purinergic signalling in autonomic control[J].Trends Neurosci,2009,32(5):241-8.
  • 4Pankratov Y,Lalo U,Krishtal O A,et al.P2X receptors and synaptic plasticity[J].Neuroscience,2009,158(1):137-48.
  • 5Li Z Y,Liang D,Chen L.Potential therapeutic targets for ATP-Gated P2X receptor ion channels[J].Assay Drug Dev Technol,2008,6(2):277-84.
  • 6Erlinge D,Burnstock G.P2 receptors in cardiovascular regulation and disease[J].Purinergic Signal,2008,4(1):1-20.
  • 7Richler E,Chaumont S,Shigetomi E,et al.Tracking transmitter-gated P2X cation channel activation in vitro and in vivo[J].Nat Methods,2008,5(1):87-93.
  • 8Li M,Chang T H,Silberberg S D,et al.Gating the pore of P2X receptor channels[J].Nat Neurosci,2008,11(8):883-7.
  • 9Pfleger K D,Eidne K A.New technologies:bioluminescence resonance energy transfer (BRET) for the detection of real time interactions involving G-protein coupled receptors[J].Pituitary,2003,6(3):141-51.
  • 10Pfleger K D,Eidne K A.Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET)[J].Nat Methods,2006,3(3):165-74.

二级参考文献28

  • 1Jorgenson R.Altered gene expression in plants due to trans interactions between homologous genes[J].Trends Biotechnol,1990,8(12):340-4.
  • 2Guo S,Kemphues K J.Par-1,a gene required for establishing polarity in C.elegans embryos,encodes a putative Ser/Thr kinase that is asymmetrically distributed[J].Cell,1995,81(4):611-20.
  • 3Fire A,Xu S,Montgomery M K,et al.Potent and specific genetic interference by double-stranded RNA in caenorhabditis elegans[J].Nature,1998,391(6669):806-11.
  • 4Stark G R,Kerr I M,Williams B R,et al.How cells respond to interferons[J].Annu Rev Biochem,1998,67:227-64.
  • 5Elbashir S M,Harborth J,Lendeckel W,et al.Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells[J].Nature,2001,411(6836):494-8.
  • 6Paul C P,Good P D,Winer I,Engelke D R.Effective expression of small interfering RNA in human cells[J].Nat Biotechnol,2002,20(5):505-8.
  • 7Zamore P D.Ancient pathways programmed by small RNAs[J].Science,2002,296(5571):1265-9.
  • 8Lee Y,Ahn C,Han J,et al.The nuclear RNase Ⅲ Drosha initiates microRNA processing[J].Nature,2003,425(6956):415-9.
  • 9Bohnsack M T,Czaplinski K,Gorlich D.Exportin 5 is a RanGTP-dependent dsRNA-binding protein that mediates nuclear export of pre-miRNAs[J].RNA,2004,10(2):185-91.
  • 10Carmell M A,Hannon G J.RNase Ⅲ enzyme and the initiation of gene silencing[J].Nat Struct Mol Biol,2004,11(3):214-8.

共引文献20

同被引文献13

  • 1刘红娟,王文宇,孙宏晨.鼠原发性三叉神经痛下丘脑β内啡肽含量测定[J].中华口腔医学杂志,2005,40(2):154-155. 被引量：5
  • 2谷远征,殷光甫,关兵才,李之望.大鼠三叉神经节神经元膜P2X嘌呤受体的特征[J].生理学报,2006,58(2):164-170. 被引量：4
  • 3李桂林,梁尚栋,徐昌水,高云,许宝华,张春平.颈上交感神经节细胞P2X_3受体介导心肌痛伤害性信息作用的电生理研究[J].中国药理学通报,2007,23(2):193-198. 被引量：4
  • 4高云,梁尚栋,邵立健,穆松牛,徐昌水,张春平.川芎嗪对P2X_3受体介导的神经病理痛的作用研究[J].中国药理学通报,2007,23(4):458-462. 被引量：9
  • 5Morell M,Ventura S,Aviles F X,et al.Protein complementation assays:approaches for the in vivo analysis of protein interactions[J].FEBS Lett,2009,583(11):1684-91.
  • 6Rose R H,Briddon S J,Holliday N D.Bimolecular fluorescence complementation:lighting up seven transmembrane domain receptor signalling networks[J].Br J Pharmacol,2009,159(4):738-50.
  • 7Shyu Y J,Suarez C D,Hu C D.Visualization of ternary complexes in living cells by using a BiFC-Based FRET assay[J].Nat Protoc,2008,3(11):1693-702.
  • 8Hu C D,Kerppola T K.Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis[J].Nat Biotechnol,2003,21(5):539-45.
  • 9Bakayan A,Vaquero C F,Picazo F,et al.Red fluorescent protein-aequorin fusions as improved bioluminescent Ca reporters in single cells and mice[J].PLoS One,2011,6(5):e19520.
  • 10Ayoub M A,Trinquet E,Pfleger K D,Pin J P.Differential association modes of the thrombin receptor PAR1 with Galphai1,Galpha12,and beta-arrestin 1[J].FASEB J,2010,24(9):3522-35.

引证文献4

二级引证文献54

中国药理学通报
2010年 第7期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部