摘要
目的：通过研究细胞因子和泌乳素（ＰＲＬ）对球后成纤维细胞（ＲＦｓ）表面细胞间粘附分子－１（ＩＣＡＭ－１）表达的影响，进一步了解甲状腺相关眼病（ＴＡＯ）的发病机理，以探索新的防治途径。方法：将体外培养的正常人ＲＦｓ与ＩＦＮ－γ、ＩＬ－４、ＩＦＮ－α和ＰＲＬ孵育７２ｈ，利用直接免疫荧光技术和流式细胞仪检测其表面ＩＣＡＭ－１表达的情况。结果：正常人ＲＦｓ中只有极少数细胞自发并较弱地表达ＩＣＡＭ－１，基础状态下的阳性百分数为（３．４９±１．９９），平均荧光强度为（４４．８４±２０．５９）；而ＩＦＮ－γ、ＩＬ－４、ＩＦＮ－α能以剂量依赖方式显著增强ＩＣＡＭ－１的表达（Ｐ＜０．０５）。ＰＲＬ不仅可以直接刺激ＲＦｓ表面ＩＣＡＭ－１的表达（Ｐ＜０．０５），其剂量相关曲线呈双相型，还能拮抗或协同ＩＦＮ－γ（１００ｕ／ｍｌ）、ＩＦＮ－α（１０００ｕ／ｍｌ）或ＩＬ－４（２．５ｎｇ／ｍｌ）的诱导作用。结论：ＩＦＮ－γ、ＩＬ－４、ＩＦ－α和ＰＲＬ对正常人ＲＦｓ表面ＩＣＡＭ－１的表达有明显的调节作用，它们可能是启动或加重ＴＡＯ自身免疫反应的重要因素。
Objective: To further understand the pathogenesis of thyroid associated ophthalmopathy and develop new curative ways, we studied the influences of cytokines and prolactin (PRL) on ICAM-1 expression on the surfaces of retroocular fibroblasts (RFs). Methods: With direct immunofluorescence technique and flowcytometry, we determined ICAM-1 expression on the surfaces of cultured normal human RFs incubated with IFN γ,IL 4,IFN α and(or) PRL for 72 hours. Results: Very few RFs spontaneously and weakly expressed ICAM 1, IFN γ,IL 4 and IFN α significantly enhanced the expression of ICAM 1 in dose-dependent manners ( P <0 05). In addition, PRL could not only directly stimulate ICAM 1 expression on the surfacts of RFs ( P <0 05), which dose-dependent curve showed “inverted U shape”, but also antagonize the effects of IFN γ (100 u/ml) as well as IFN α (1000 u/ml) and coact with IL 4 (2.5 ng/ml). Conclusion: IFN γ, IL 4, IFN α and PRL played significant roles in regulating ICAM 1 expression on the surfaces of normal human RFs and they might be the important factors for initiating or promoting autoimmune reactions in thyroid associated ophthalmopathy.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1999年第2期101-104,共4页
Journal of China Medical University
基金
国家自然科学基金
关键词
甲状腺相关眼病
细胞因子
泌乳素
RFS
ICAM-1
thyroid associated ophthalmopathy
cytokine
prolactin
retroocular fibroblast
intercellular adhesion molecule 1
