摘要
探讨灵芝多糖对小鼠腹腔巨噬细胞，肿瘤坏死因子α，脾细胞γ干扰素的产生及其ｍＲＮＡ表达的影响。方法；采集小鼠ＰＭ和脾淋巴细胞，体外给药，分离细胞培养上清。生物法测定ＴＮＦα，ＥＬＩＳＡ测定ＩＦＮγ；ＲＴ－ＰＣＲ方法检测ｍＲＮＡ的表达。结果；ＧＬ－Ｂ２５－４００ｍｇ．Ｌ＾－１使正常小鼠ＰＭ培养上清中ＴＮＦα活性升高，并呈剂量依赖关系，２４ｈ在高峰，７２ｈ后开始减少。
To investigate the effects of Ganoderma lucidum polysaccharides B（GL-B）, on production of tumor necrosis factor alpha （TNFα）, interferon γ（IFNγ） and their mRNA expression in murine peritoneal macrophage （PM） and splenocytes. Methods: Mouse PM and splenocytes were treated in vitro with GL-B, and cell culture supernatant was separated. Both TNFα and IFNγ were detected by biological assay, and ELISA respectively. The level of mRNA was detected with the method of reverse transcript ion- polymerase chain reaction （RT- PCR ）. Results: TNFα production w as dose-dependently increased in the supernatant of normal murine PM by the effect of GL-B 25-400 mg ·L-1. The maximal effect was observed after 24 h of incubation and decreased after 72 h. GL-B significantly promoted IFNγ production in normal murine splenocytes with the dose of 12. 5-200 mg ·L-1. The maximal effect was observed after 24 h of incubation and decreased after 48 h. GL-B significantly promoted IFNγ mRNA expression in normal murine splenocytes with the dose of 50, 100 and 200 mg· L-1. Conclusion: GL-B significantly promoted TNFα, IFNγ mRNA expression in normal murine PM, and splenocytes, thus increased TNFα, IFNγ production.
出处
《北京医科大学学报》
CSCD
1999年第2期179-183,共5页
Journal of Peking University(Health Sciences)
基金
中国博士后基金
关键词
灵芝多糖
药理学
免疫增强剂
TNFΑ
干扰素Γ
Ganoderma lucidum/pharmacol Polysaccharides/ pharmacol Tumor necrosis factor/ secret Interferon gamma
recombinant/secret RNA
messenger/metab
