摘要
目的检测血红蛋白H病(HbH)患儿血清转铁蛋白受体(sTfR)及血清铁蛋白(SF)质量浓度,了解HbH病患儿体内铁负荷状况,并应用ROC曲线对sTfR及其复合参数sTfR/lgSF进行分析比较,得出判断HbH病患儿体内铁负荷状况较可靠的指标。方法采用ELISA法检测50例HbH病患儿(病例组)及30例健康对照者(健康对照组)血浆sTfR及SF质量浓度,通过计算分别得出2组sTfR/lgSF值;利用ROC曲线,以SF作为金标准对sTfR/lgSF、sTfR这2个参数在诊断HbH病患儿体内是否存在铁超负荷状况时的价值进行分析。结果病例组sTfR质量浓度为(6.74±1.02)mg.L-1,健康对照组sTfR质量浓度为(8.09±0.67)mg.L-1,病例组43例(86%)患儿sTfR质量浓度低于健康对照组,差异有统计学意义(P<0.01),余7例sTfR质量浓度在8.0mg.L-1以上,接近正常值;病例组SF质量浓度为(203.19±84.77)μg.L-1,健康对照组SF质量浓度为(84.13±83.03)μg.L-1,病例组SF水平较健康对照组增高,差异有统计学意义(P<0.01),其中43例已达铁超负荷诊断标准;病例组sTfR/lgSF水平为3.05±0.67,对照组为4.43±1.11,病例组sTfR/lgSF较对照组降低,差异有统计学意义(P<0.01);ROC曲线下面积sTfR/lgSF为0.871,sTfR为0.661。结论 HbH病患儿发生铁超负荷的危险性较大;sTfR/lgSF是判断HbH患儿体内铁超负荷较可靠的指标。
Objective To measure the levels of serum transferrin receptors （sTfR） and serum ferritin （SF） in children with hemoglobin H disease for investigation the iron metabolism（sideropenic or iron overload） and to guide the proper intervention.To get a best index of judging the iron overload in HbH children by analysis of the values of sTfR and sTfR/lgSF.Methods Peripheral blood specimens were obtained from 50 cases of HbH children and 30 cases of healthy normal controls,the levels of sTfR and SF were examined by enzyme linked immunosorbent assays（ELISA）.The diagnosis efficiency of sTfR and sTfR/lgSF in HbH children′s iron metabolism was judged by receiver operator cha-racteristic （ROC） curve analysis.Results The level of sTfR in HbH group was （6.74±1.02） mg·L^-1,which was significantly lower than that in healthy control group[（8.09±0.67） mg·L^-1]（P〈0.01）,except 7 children;the level of SF in HbH group was （203.19±84.77） μg·L^-1which was significantly higher than that in healthy control group[（84.13±83.03） μg·L^-1（P〈0.01）,iron overload was found in 43 children according to the diagnostic criteria;the sTfR/lgSF value in HbH children group（3.05±0.67） was significantly lower than that in healthy control group（4.43±1.11）（P〈0.01）;the area of ROC,sTfR/lgSF value and sTfR value were 0.871 and 0.661,respectively.Conclusions There are iron overload in the part of the HbH children;the sTfR/lgSF value is the better index to determine iron status in HbH children.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2010年第15期1153-1154,1181,共3页
Journal of Applied Clinical Pediatrics
关键词
血红蛋白H病
血清转铁蛋白受体
铁负荷
hemoglobin H disease
serum transferrin receptors
iron overload
