摘要
本研究旨在研究日粮中等硫添加DL-蛋氨酸(DL-Met)和蛋氨酸羟基类似物游离酸(MHA-FA)对幼建鲤生长性能、消化吸收酶活性和抗氧化指标的影响。选择平均体重为(8.24±0.03)g的健康幼建鲤300尾,随机分成2组,每组3个重复,每个重复50尾,分别饲喂等硫的DL-Met和MHA-FA的实用日粮,试验期60d。结果表明:MHA-FA组的增重、饲料转化率以及蛋白质、脂肪和灰分的沉积率与DL-Met组差异不显著(P>0.05),但摄食量显著低于DL-Met组(P<0.05)。MHA-FA组的肌肉和肝胰脏中谷草转氨酶(GOT)和谷丙转氨酶(GPT)活力以及血浆氨含量与DL-Met组差异不显著(P>0.05)。MHA-FA组的肝胰脏和肠道胰蛋白酶、糜蛋白酶以及肠道脂肪酶和淀粉酶活力与DL-Met组均差异不显著(P>0.05),但肝胰脏脂肪酶和淀粉酶活力显著低于DL-Met组(P<0.05)。MHA-FA组的前肠和中肠碱性磷酸酶(AKP)、各肠段Na+,K+-ATP酶、中肠γ-谷氨酰转肽酶(γ-GT)以及全肠肌酸激酶(CK)活力与DL-Met组均差异不显著(P>0.05),但后肠AKP、前肠和后肠γ-GT活力显著低于DL-Met组(P<0.05)。与DL-Met组相比,MHA-FA组的血清、肠道和肝胰脏丙二醛(MDA)含量显著升高(P<0.05),血清谷胱甘肽(GSH)、血清和肠道过氧化氢酶(CAT)、肠道和肝胰脏谷胱甘肽硫转移酶(GST)、谷胱甘肽过氧化物酶(GSH-Px)活力显著降低(P<0.05)。综上所述,幼建鲤基础日粮中等硫添加MHA-FA基本可以达到与DL-Met相同的饲喂效果。
The purpose of this study was to study the effects of equal-sulfur DL-methionine（DL-Met） and methionine hydroxy analogue free acid（MHA-FA） supplementation in diets on growth performance,activities of digestive and absorptive enzymes and antioxidation indices of juvenile Jian carp（Cyprinus carpio var.Jian）.A total of 450 fish with average initial weight of（8.24±0.03） g were randomly divided into 2 groups with 3 replicates in each group and 10 fish per replicate.The fish of two groups were fed practical diets supplemented with DL-Met and MHA-FA on equal-sulfur basis,respectively.The trial lasted for 60 days.The results showed as follows：MHA-FA and DL-Met had the same effects on weight gain,feed conversion ratio,productive values of protein,fat and ash in juvenile Jian carp（P0.05）,but the feed intake in MHA-FA group was significantly lower than that in DL-Met group（P0.05）.Glutamate-oxaloacetate transaminase（GOT） and glutamate-pyruvate transaminase（GPT） activities in muscle and hepatopancreas and plasma ammonia content also had no significantly difference between MHA-FA and DL-Met groups（P0.05）.The activities of trypsin and amylase in intestine and hepatopancreas,lipase and amylase in intestine were not significant different between MHA-FA and DL-Met groups（P0.05）,while the activities of lipase and amylase in hepatopancreas in MHA-FA group were significantly lower than those in DL-Met group（P0.05）.The activities of alkaline phosphatase（AKP） in foregut and midgut,Na＋,K＋-ATPase in foregut,midgut and hindgut,γ-glutamyl transpeptidase（γ-GT） in midgut and creatinekinase（CK） in whole intestine were not significantly different between MHA-FA and DL-Met groups（P0.05）.However,the activities of AKP in hindgut,γ-GT in foregut and hindgut in MHA-FA group were significantly lower than those in DL-Met group（P0.05）.Compared with the DL-Met group,the content of MDA in serum,intestine and hepatopancreas in MHA-FA group was significantly higher（P0.05）,while the activities of glutathione（GSH） in serum,catalase（CAT） in serum and intestine,glutathione S-transferase（GST）,glutathione peroxidase（GSH-Px） in intestine and hepatopancreas in MHA-FA group were significantly lower（P0.05）.In conclusion,supplementation of MHA-FA and DL-Met on equal-sulfur basis in juvenile Jian carp diet nearly obtained the same feeding effects.
出处
《动物营养学报》
CAS
CSCD
北大核心
2010年第4期1122-1130,共9页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
教育部长江学者和创新团队发展计划(IRTO555)
