摘要
为了探明野生和人工养殖褐牙鲆亲鱼的繁育性能和卵巢发育之间的关系,采用生物化学方法,将处于Ⅲ～Ⅳ期和Ⅴ期的野生和养殖褐牙鲆亲鱼中肌肉、肝脏及卵巢氨基酸含量分别进行测定,结果表明,(1)褐牙鲆肌肉、肝脏、卵巢3种组织器官中总氨基酸含量大小关系为肌肉>卵巢>肝脏;(2)发育同期的野生和养殖亲鱼肌肉中大部分氨基酸含量差异不显著(P>0.05),并且随着性腺的成熟,野生和养殖亲鱼肌肉中大部分氨基酸较为稳定,其中含量变化显著的必需氨基酸为蛋氨酸、组氨酸(P<0.05);(3)发育同期的养殖亲鱼肝脏中大部分氨基酸含量高于野生亲鱼,随着性腺发育,野生和养殖亲鱼肝脏中氨基酸含量呈明显上升趋势;(4)在Ⅴ期卵巢氨基酸组成中,除蛋氨酸、组氨酸含量存在显著差异外,野生和养殖亲鱼卵巢中氨基酸总量及必需氨基酸总量均不存在显著差异(P>0.05),同时,随着性腺发育,野生和养殖亲鱼卵巢中氨基酸含量呈明显上升趋势,差异显著的必需氨基酸有精氨酸、蛋氨酸、组氨酸(P<0.05)。
Amino acid composition in muscle,liver and ovary of the wild and cultured Paralichthys olivaceus broodstock at stages Ⅲ-Ⅳ and Ⅴ was determined by biochemical methods,to ascertain their effects on the reproductive performance.The results indicated that the total amino acid concentration of muscle,liver and ovary of both the wild and the cultured broodstock is as follows： muscle ovary liver;There were no significant differences in most amino acid concentration of muscle between the wild and the cultured broodstock.The muscle of the wild and the cultured broodstock have constant amino acid composition with development of the ovary except Methionine and Histidine;The most amino acid concentration in liver of the cultured broodstock were higher than the same-phased wild broodstock,and the amino acid concentration in liver of both the wild and the cultured broodstock increased with the development;There were no significant differences of total amino acid concentration and essential amino acid concentration in ovary between the wild broodstock and the same-phased cultured broodstock,but Methionine concentration of the wild broodstock was higher than the cultured one.The amino acid concentration in different tissues of the wild and the cultured broodstock increased with the development especially Arginine,Methionine,Histidine.Arginine,Methionine,Histidine were presumed to induce spawning differences between the wild broodstock and the cultured one,so further researches are needed.
出处
《水产学报》
CAS
CSCD
北大核心
2010年第11期1736-1743,共8页
Journal of Fisheries of China
基金
国家重点行业公益项目(农业部
nyhyzx07-046)
山东省水生动物营养与饲料泰山学者岗位经费资助
关键词
褐牙鲆
肌肉
肝脏
卵巢
氨基酸组成
Paralichthys olivaceus
muscle
liver
ovary
amino acid composition
