摘要
目的探讨染锰大鼠血清生殖激素水平与生精细胞凋亡基因Caspase-3表达关系。方法雄性SD大鼠48只,随机分为对照组和15,30 mg/kg氯化锰组,分别染锰4,6周,乙醚麻醉,眼眶取血,分离双侧睾丸,称重,采用免疫组织化学方法检测生精细胞caspase-3表达,放射免疫法检测血清睾酮(testosterone,T)、卵泡刺激素(follicle stimu-lating,FSH)、黄体生成素(luteinizine hormone,LH)水平。结果 30 mg/kg氯化锰组染锰4,6周,血清中T、FSH、LH含量分别为(2.78±0.74)和(2.76±0.81)nmol/L,(0.44±0.03)和(0.57±0.03),(0.19±0.02)和(0.19±0.03)U/L;Caspase-3表达水平分别为(65.49±11.34)和(82.65±5.26);染锰组大鼠生精细胞Caspase-3表达与血清T含量呈负相关(r=-0.799,P<0.01),与血清中FSH、LH水平呈正相关(r=0.735,r=0.737,P<0.01)。结论染锰大鼠血清T水平降低,FSH和LH水平上升是生精细胞caspase-3表达上调的主要原因。
Objective To study the relationship between serum reproductive hormone level and expression of caspase-3 in spermatogenic cell in rats with manganese exposure.Methods Forty-eight male rats were randomly divided into one control group and two manganese chloride（MnCl2）（15,30 mg/kg） exposure groups.After exposed to manganese for 4 and 6 weeks,the serum testosterone（T）,follicle stimulating bormone（FSH）,and luteinizine hormone（LH） levels were detected with radioimmunoassay.The expression of caspase-3 in spermatogenic cells was detected with immunohistochemical methods（SABC）.Results The levels of T,FSH and LH in 30 mg/kg MnCl2 groups were 2.78±0.74 nmol/L,0.44±0.03 U/L,0.19±0.02 U/L for rats wtih 4 weeks＇ exposure and 2.76±0.81 nmol/L,0.57±0.03 U/L,0.19±0.02 U/L for rats with 6 weeks＇ exposure,respectively.The percentages of caspase-3-positive cell were 65.49±11.34 and 82.65±5.26 for rats with 4 weeks and 6 weeks of manganses exposure,respectively.There existed a negative correlation between the caspase-3-positive-cell rate and serum level of T（r=-0.799,P0.01）.There existed a positive correlation between the caspase-3-positive-cell rate and serum levels of FSH and LH（r=0.735,P0.01;r=0.737,P0.01）.Conclusion The decrease of serum T and the increases of serum FSH and LH may be the important causes of the up-regulation of caspase-3 expression in spermatogenic cells in rats exposed to manganese.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2010年第8期1064-1065,共2页
Chinese Journal of Public Health
基金
合肥市科技基金(遵市科合社字200707号)
