摘要
目的探讨医院临床分离的超广谱β-内酰胺酶阳性肺炎克雷伯菌产碳青酶烯酶的情况及对产碳青酶烯酶菌株进行分析,为临床合理选用抗菌药物提供依据。方法收集196株临床分离超广谱β-内酰胺酶阳性的肺炎克雷伯菌,通过改良Hodge法检测其碳青酶烯酶,用纸片琼脂扩散法和VITEK-AMS 60全自动微生物分析仪进行药物敏感性试验。结果 196株超广谱β-内酰胺酶阳性的肺炎克雷伯菌中检出1株产碳青酶烯酶菌株,该菌株对亚胺培南和美罗培南在内的碳青酶烯类、青霉素类、头孢菌素类抗菌药物耐药。结论通过改良Hodge法检测碳青酶烯酶操作简单,结果易判读,且准确性高,适合临床微生物实验室常规采用。
OBJECTIVE To investigate the production and resistance of Klebsiella pneumoniae carbapenemase（KPC） in extended spectrum β-lactamases-producing K.pneumoniae in hospital.METHODS A total of 196 clinical strains of extended spectrum β-lactamases-producing K.pneumoniae were collected from our hospital.The modified Hodge test was used to detect KPC.Kirby-Bauer method and VITEK-AMS 60 were performed to detect the sensitivity of the antimicrobial agents against the K.pneumoniae.RESULTS There was one strain of KPC producing K.pneumoniae from 196 strains.The KPC producing strain was resistant to carbapenems including imipenem and meropenem,penicillins and cephalosporins antibiotics.CONCLUSIONS Using modified Hodge test to detect KPC is simple,with easy interpretation of results,and high accuracy for routine clinical microbiology laboratory detection.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2010年第15期2324-2326,共3页
Chinese Journal of Nosocomiology
