摘要
本文以甘蓝型冬油菜为料材,对游离小孢子培养诱导胚状体成苗进行了研究。结果表明游离小孢子培养25天就可见到多种类型的胚状体;振荡培养有利于胚状体的生长发育,子叶型胚频率较高;在相同培养条件下,游离小孢子胚状体成苗频率取决于胚状体的类型。本文还对花粉小孢子胚的发生、分化作了较详细的形态学观察。
Using Brassica napus L. as experimental materials, we studied the plant regeneration through embryoids in isolated mierospore culture. The result showed that embryoids of several types occurred through 25 days of mierospore culture and that the shaking culture method was advantageous to the growth and development of embryoids. Also, the plant regeneration frequency from embryoids in isolated microspore culture was observed to be determined by embryoid type under the same culture condition. In addition, the detailed morphological observations on the occurrence and differentiation of embryoids in the isolated microspore culture were carried out.
出处
《上海农业学报》
CSCD
1990年第4期11-16,共6页
Acta Agriculturae Shanghai
关键词
油菜
甘蓝型
游离小孢子
胚状体
Brassica napus L., Isolated microspore, Shaking culture, Embryoid, Plant regeneration
