HL-60细胞培养上清液对人脐带血单个核细胞增殖的影响被引量：1

Effects of HL-60 cell supernatant on proliferation of mononulear cells from human umbilical cord blood

导出

摘要目的白血病细胞能够通过自分泌方式分泌细胞因子而作用于自身,使细胞大量的增殖;白血病细胞所分泌的细胞因子也能作用于人脐带血(UCB)单个核细胞(MNCs)中的CD34+/CD133+细胞亚群使之增殖。关于HL-60细胞(急性早幼粒白血病细胞)培养上清液能否使UCB-MNCs增殖尚未见报道,本实验主要目的是观察HL-60细胞培养上清液对人UCB-MNCs增殖的影响。方法实验分为①有HL-60细胞培养上清液+StemSpanSFEM组;②StemSpan誖SFEM组;③HL-60细胞培养上清液+高糖(HG)-DMEM组;④HG-DMEM组;⑤HL-60细胞培养上清液+低糖(LG)-DMEM组;⑥LG-DMEM组。收分离的人UCB-MNCs按2.5×106/瓶分别接种于6组培养液中,37℃,5%CO2,饱和湿度培养,分别于第3,第6,第9,第12天计细胞总数。结果①培养至第3天,HL-60细胞培养上清液+StemSpanSFEM组和StemSpanSFEM组MNCs数较培养前和其余各组明显升高(均P<0.05),至第9天细胞数维持在较高水平;②HL-60细胞培养上清液+StemSpanSFEM组和StemSpanSFEM组各时点上,前者MNCs数量较后者有所增加,但无统计学意义(P>0.05);③添加HL-60上清的高糖组和低糖组,各时点上的MNCs数均较相应的对照组升高(均P<0.05)。结论 HL-60细胞培养上清液对人UCB-MNCs具有一定的增殖作用,其有效生物活性物质有待进一步研究。 Objective Leukemic cells can excrete cytokines by an autocrine way,which contribute to its large-scale proliferation,and the cytokines may also contribute to the proliferations of CD34＋/CD133＋ subpopulation of UCB-MNCs too.To the effect of HL-60 cell supernatant on the proliferation of UCB-MNCs,there is not related report so far.The aim of this study is to investigate effects of HL-60 cell supernatant on proliferations of mononuclear cells from human umbilical cord blood.Methods Six various cultural system are listed as follows： ① HL-60 cell supernatant＋ StemSpanSFEM;② StemSpanSFEM;③ HL-60 cell supernatant＋ HG-DMEM;④ HG-DMEM;⑤ HL-60 cell supernatant LG-DMEM;⑥ LG-DMEM.2.50×106 of UCB-MNCs per bottle with the above each cultural system is incubated at 37℃ in a 5%CO2,saturation humidity of incubator,the number of cell each group calculated by trypan blue assay on 3rd,6th,9th,and 12th day.Results ① On 3rd day,the numbers of MNCs,both HL-60 cell supernatant＋StemSpanSFEM and StemSpanSFEM groups are increased significantly than other groups’（all P0.05）,and kept a steady cell number.② As compared to HL-60 cell supernatant＋StemSpanSFEM and StemSpanSFEM groups,the number of MNCs of former is higher at any time,but no statistically significant（P0.05）;③ Number of MNCs,both in HG-DMEM and LG-DMEM medium with HL-60 cell supernatant,are higher than control group without HL-60 cell supernatant（all P 0.05）.Conclusions HL-60 cell supernatants may contribute to the proliferation of UCB-MNCs,and its related bioactive compound need further investigation in the near future..

作者郑毅陈代雄

机构地区合肥医学院附属医院贵州省重点细胞工程实验室合肥医药高等专科学校生理教研室

出处《合肥医学院学报》 2010年第2期125-127,共3页 Journal of Zunyi Medical University

关键词 HL-60细胞上清液单个核细胞增殖 HL-60 cell supernatant mononuclear cell proliferation

分类号 R739.8 [医药卫生—肿瘤]

引文网络
相关文献

参考文献4

1Young Diane C,Griffin James D.Autocrine Secretion of GM-CSF in Acute Myeloblastic Leukemia[J].Blood,1986,68:1178-1181.
2樊娟,徐功立,王春霞,徐键,马兰英.急性髓性白血病细胞自分泌造血生长因子水平及其临床意义[J].中国免疫学杂志,2000,16(5):283-283. 被引量：3
3H Sugiyama,K Inoue,H Ogawa,et al.The expression of IL-6 and its related genes in acute leukemia[J].Leuk Lymphoma,1996,21(1-2):49-52.
4Estrov Zeev,Meir Roza,Barak Yigal,et al.Human Growth Hormone and Insulin-like Growth Factor-1 Enhance the Proliferation of Human Leukemic Blasts[J].Journal of Clinical Oncology,1991,9(3):394-399.