摘要
针对芒果炭疽病菌进行了拮抗菌的分离和筛选,并且采用有效菌株的发酵液进行了平板培养基抑菌试验以及采后芒果果实炭疽病防病试验。结果从24个菌株中筛选得到1株有效抑制炭疽病菌的芽孢杆菌M35,其抑菌圈大于13mm,而且其发酵液对炭疽病菌也有明显的抑制作用;果实防病试验结果也显示,该菌株对于芒果采后炭疽病有显著的防治效果,且随着该拮抗菌菌悬液浓度的增加,防病效果提高,在浓度为l×l08CFU/mL时,发病率为45%,病斑直径为14.1mm。
Twenty-four strains were isolated from leaves and fruits of mango and were cultured to screen high resistant strains. Among the strains, 6 showed higher inhibition effect against Colletotrichum gloeosporioides than the control, of which the strain M35 with 13 mm inhibition zone was the most effec- tive one. The free-cell fermentation of M35 strain also showed very effectively resistant to pathogen. The experiment on mango showed that the inhibitory rate increased gradually with rising cell concentration of M35. The incidence was just 45% and the diameter of spot was 14.1 mm when the cell concentration was 1 ×10^8 CFU/mL.
出处
《热带农业科学》
2010年第5期12-14,共3页
Chinese Journal of Tropical Agriculture
基金
中国热带农业科学院中央级公益性科研院所基本科研业务费专项(No.sscri200711)
海南省自然科学基金(No.808183)项目
关键词
拮抗菌
分离
筛选
防病效果
antagonists
isolation
selection
control effect
