摘要
【目的】本试验旨在研究母鸡采食量限饲对子代脂肪沉积、脂肪酸合成酶(fattyacid synthetase,FAS)活性及其基因表达的影响,探讨母体营养效应及子代后天的补偿作用。【方法】选择东北农业大学繁育的高、低脂系肉种母鸡各384羽,分别分为两个处理组。产蛋期,处理一饲喂正常日粮,处理二日采食量是处理一的75%。产蛋高峰期收集种蛋进行孵化。子代自由采食常规水平日粮。【结果】母鸡采食量限饲显著提高子代28日龄腿肌脂肪含量(P<0.05),56日龄时差异不显著(P>0.05);子代肝脏脂肪含量品系间存在差异,高脂系显著高于低脂系(P<0.05);品系与采食水平的互作对子代肝脏脂肪含量和28日龄胸肌脂肪含量影响显著(P<0.05);子代腹脂率品系间差异极显著(P<0.01)。56日龄时,子代肝脏FAS酶活性品系间差异显著(P<0.05),品系与饲喂水平的互作对肝脏FAS酶活性影响显著(P<0.05)。子代肝脏FAS mRNA表达丰度1日龄时品系间差异显著(P<0.05);28日龄时,品系与饲喂水平的互作对肝脏FAS mRNA的表达影响显著(P<0.05);母鸡采食量限饲显著提高了28日龄子代肝脏FAS mRNA的表达丰度(P<0.05);56日龄时差异不显著(P>0.05)。【结论】母鸡采食量限饲影响子代脂肪的沉积,且品系间表现出不同的趋势;56日龄时,肉仔鸡肝脏FAS酶活性品系间差异显著(P<0.05),且品系与饲喂水平的交互作用影响显著(P<0.05);肉仔鸡肝脏FAS mRNA的表达丰度受母体效应的影响,并且在其后生长过程中表现出补偿作用。
【Objective】 An experiment was conducted to evaluate the effects of maternal feed restriction on offspring liver fat deposition and the liver lipid metabolism, in order to find out the effect of maternal nutrition and offspring compensation. 【Method】 Three hundred and eighty-four fat and lean line broiler breeders bred by Northeast Agricultural University were, respectively divided into two treatments. During the laying period, treatment one was fed normally, and the daily feed intake of treatment two was 75% of treatment one. The eggs were collected and hatched when the laying rate rose to the peak. The offspring were given ad libitum access to standard diets until 56 days. 【Result】 The results showed that the maternal feed restriction significantly increased the thigh muscle fat rate of 28 day （P〈0.05）, the difference at 56 day was not significant （P〉0.05）. Strain had a significant effect on the liver fat rate （P〈0.05）, the fat line was higher than lean line （P〈0.05）. There was a significant interaction between strain and feed level on the liver fat rate and 28 day breast muscle fat rate （P〈0.05）. The abdominal fat percentage between two strains was different significantly （P〈0.01）. At 56 day , strain had a significant effect on the fatty acid synthetase （FAS） activity, and the interaction effect between the strain and feed level was significant （P〈0.05）. The expression of liver FAS gene of 1 day was affected significantly by strain （P0〈.05）, the effect of the interaction between the strain and feed level on the expression of liver FAS gene of 28 days wassignificant （P〈0.05）. The maternal feed restriction significantly increased the expression of liver FAS gene of 28 days （P〈0.05）, the difference at 56 days was not significant （P〉0.05）. 【Conclusion】 The results demonstrated that the maternal feed restriction had effects on the fat deposition, and the trend between the two strains was different. The strain had a significant effect on the FAS activity at 56 days （P〈0.05）, and the interaction effect between the strain and feed level was significant （P〈0.05）. Maternal effect on the liver FAS mRNA expression and the offspring compensation were observed in the offspring.
出处
《中国农业科学》
CAS
CSCD
北大核心
2010年第15期3230-3236,共7页
Scientia Agricultura Sinica
基金
国家重点基础研究发展计划项目(2004CB117505)
东北农业大学创新团队项目(CXT006)
关键词
采食量限饲
高、低脂系
肉仔鸡
脂肪沉积
脂肪酸合成酶
feed restriction
fat and lean line
broiler breeder
fat deposition
fatty acid synthetase
