摘要
采用超临界流体萃取法（ＳＦＥ）提取中药何首乌中的蒽醌类成分，并用ＲＰ－ＨＰＬＣ法进行分离测定。在 ＳＦＥ中，通过对萃取条件的考察，确定萃取压力为 ４２ ＭＰａ，温度 ７０℃，改性剂量 ０．４ｍＬ，静态萃取时间 ５ｍｉｎ及动态萃取体积 ５ｍＬ。 ＲＰ－ＨＰＬＣ中，以Ｃ_８为固定相，甲醇－０． ２％磷酸溶液（７０：３０）为流动相，检测波长 ４３７ｎｍ。本法准确可靠，何首乌中各成分的平均回收率分别为大黄酸９９．１％（ＲＳＤ ２．０％）、大黄素 ９８．２％（ＲＳＤ１．１％）、大黄酚 １００．７％（ＲＳＤ ２．６％）和大黄素甲醚９９．５％（ＲＳＤ ０．９％）。
Supercritical fluid extraction (SFE) was used to extract anthraquinones in Polygonum multiflorum Thunb. and contents of rhein, emodin and physcion in the extract were determined by RP-HPLC. SFE conditions: pressure 42 MPa, temperature 70℃, 0. 4 mL methanol as modifier, static extraction 5 min and dynamic extraction volume 5 mL. HPLC conditions: the HPLC column was packed with C_8 (5 um); the mobile phase was methanol: 0. 2% phosphoric acid (70:30); the detection wave-length was 437 nm. The method was accurate and reliable. The mean recovery was 99.1% (RSD 2.0%) for rhein, 98.2% (RSD 1.1 % ) for emodin, 100. 7% (RSD 2.6% ) for chrysophanol and 99.5% (RSD 0.9% ) for physcion.
出处
《中草药》
CAS
CSCD
北大核心
1999年第4期258-260,共3页
Chinese Traditional and Herbal Drugs
关键词
超临界流体萃取
HPLC
何首乌
蒽醌类成分
大黄酸
Ploygonum multiflorum Thunb. supercritical fluid extraction (SFE)
HPLC anthraquinones
