摘要
目的：探讨ＶＥＧＦ在固体肿瘤生长、进展、转移过程中的作用为通过阻断ＶＥＧＦ与其受体ＫＤＲ的作用治疗肿瘤提供依据．方法：采用免疫组织化学ＡＢＣ法对５０例人胃癌组织中的血管内皮生长因子（ＶＥＧＦ）及其受体（ＫＤＲ）的定位与分布进行研究．结果：５０％的胃癌组织表达ＶＥＧＦ，阳性物质主要位于肿瘤细胞胞膜及胞浆，ＫＤＲ染色阳性物质主要位于癌组织及癌组织旁的血管内皮细胞上．Ⅲ，Ⅳ期胃癌组织ＶＥＧＦ的阳性率（６６．６７％，１２／１８；８１．８２％，９／１１）均高于Ⅰ，Ⅱ期的胃癌组织（２０％，２／１０；１８．１８％，２／１１）（Ｐ＜０．０５）；不同组织类型的ＶＥＧＦ阳性表达率无差异；胃癌组织伴发淋巴结转移或肝转移的ＶＥＧＦ阳性率（６７．７４％，２１／３１；１００％，０／６）明显高于未发生转移的胃癌组织（２１．０５％，４／１９；４３．１８％，１９／４４）（Ｐ＜０．０５）；胃癌组织ＶＥＧＦ的表达与肿瘤浸润及浆膜有关．结论：ＶＥＧＦ与其受体ＫＤＲ的作用在胃癌的生长、进展和转移中可能有重要作用．
AIM: To provide the basis for preventing tumor growth by blocking the effect of vascular endothelial growth factor(VEGF), we observed the localization and distribution of VEGF and its receptor KDR to investigate the biological relevance of the VEGF/KDR ligand/receptor system for their growth and metastases. METHODS: VEGF and KDR were detected in 50 gastric carcinoma(GC) by immunohistochemical ABC technique. RESULTS: VEGFpositive rate was 50%,and VEGF was mainly localized to the cytoplasm and the membrane of the tumor cells; and KDR was mainly localized in the cytoplasm and the membrane of vascular endothelial cell in gastric cancer tissues and their related PeriCancerous tissue; the VEGFpositive rates of TNM grade (66.67%,12/18) and grade (81.82%,9/11) GC were higher than those of grade (20.00%,2/10) and grade (18.18%,2/11) GC (P<0.05); there was no significant association between VEGF expression and the histologic type; the VEGFpositive rate was significantly higher in patients with lymph nodal metastases (67.74%,21/31) or liver metastases (100.00%,6/6) than in those without such metastases(21.05%,4/19 or 43.18%,19/44)(P<0.05).Significant difference was noted with respect to serosal involvement of tumor(P<0.05). CONCLUSION: The biologic effect of VEGF/KDR system might play an important role in the growth, progression, and metastasis of solid tumors.
出处
《第四军医大学学报》
1999年第4期316-318,共3页
Journal of the Fourth Military Medical University
关键词
胃癌
VEGF
受体
免疫组织化学
gastric carcinoma
vascular endothelial growth factor
receptor
immunohistochemistry
