摘要
目的研究支气管哮喘(简称哮喘)大鼠模型支气管肺泡灌洗液(BALF)、血液、脾脏CD4+CD25+T细胞的变化,及地塞米松对CD4+CD25+T细胞的影响。方法 50只SD大鼠随机分为5组,空白对照(A)组,哮喘(B)组,地塞米松1(C)组、地塞米松2(D)组,地塞米松3(E)组。A组第1天给予腹腔注射生理盐水1ml,第15～21天每天给予生理盐水雾化。B、C、D、E组用卵蛋白建立哮喘大鼠模型,第1天,每只大鼠腹腔注射抗原1ml(卵蛋白1mg+灭活百日咳杆菌9×106个+氢氧化铝干粉100mg)混悬液,第15～21天给予1%的卵蛋白雾化30min,C、D、E组于雾化后分别给予腹腔注射地塞米松0.2mg/kg、1mg/kg、2mg/kg。采用流式细胞仪检测的方法,观察大鼠体内BALF、外周血、脾脏CD4+CD25+T细胞的变化及使用不同剂量地塞米松后对其的影响。结果 B组BALF、外周血、脾脏CD4+CD25+T细胞表达占CD4+T细胞的百分比分别是(42.21±5.62)%、(12.69±2.70)%、(11.15±1.05)%,A组结果分别是(18.76±5.85)%、(6.21±1.73)%、(7.85±2.13)%。B组与A组比较,差异均具有统计学意义(P<0.01,P<0.01,P<0.05);C组、D组、E组BALF中CD4+CD25+T细胞占CD4+T细胞的百分比表达分别是(10.49±4.03)%、(13.28±5.12)%、(7.51±5.39)%,显著低于A组和B组,(P<0.05,P<0.01);外周血中,C组(6.03±1.43)%、D组(4.88±0.95)%与A组(6.21±1.73)%比较,差异无统计学意义,E组(3.49±0.62)%与C组、A组比较,差异有统计学意义(P<0.05)。脾脏中,C组(7.25±1.82)%、D组(8.63±3.18)%与A组(7.85±2.13)%比较,差异无统计学意义,E组(3.38±1.37)%与C组、D组、A组比较,差异有统计学意义(P<0.05)。结论 CD4+CD25+T细胞在哮喘大鼠体内有明显的优势表达,可能是哮喘发病的机制之一。地塞米松可以抑制CD4+CD25+T细胞的表达。BALF内CD4+CD25+T细胞的变化与外周血和脾脏的变化具有一致性,监测外周血或脾脏CD4+CD25+T细胞变化可了解肺部情况。
Objective To investigate the change of CD4^＋ CD25^＋T cells in bronchoalvelar lavage fluid （BALF）,blood and spleen of asthmatic rat and the effect of dexamethasone on CD4^＋ CD25^＋ T cells. Methods Fifty SD rats were randomly divided into five groups with 10 rats in each group：control group （A）, asthmatic group （ B）, dexamethasone low-dose group （ C）, dexamethasone mid-dose group （D）, dexamethasone high-dose group （E）. Group A was established by saline. The rat was injected saline 1 ml in 1 day and challenged from days 15 to 21 by saline 10 ml. The group B,C,D,E,were sensitized on days 1 by injected OVA lmg and 1% OVA aerosol challenged from days 15 to 21. The group C,D,E were injected dexamethasone（0.2 mg/kg, 1 mg/kg,2 mg/kg） after challenges 30 minutes. CD4^＋ CD25^＋ T cells in BALF,blood and spleen are measured by flow cytometric analysis. Results BALF,blood and spleen＇s ratio of CD4^＋ CD25^＋ T cells to CD4^＋T cells of group B were significantly higher than those in group A [（42.21±5.62）% vs （18.76±5.85）%, P 〈0.01],[（12.69±2.70） % vs （6.27±1.73）%, P 〈0.01], [（11.15±1.05）% vs （7.85±2.13）%, P 〈0.05]. In BALF, group C （10. 49 ± 4. 03 ） % , group D （13.28±5.12）%,group E （7.51 ±5.3）%, compared with group A（18.76 ± 5.85）% group B （42.21± 5.62） %, the differences were significant （all P 〈 0.05 ）. In blood, group C （6.03 ± 1.43 ） %, group D（4. 88±0. 95） %, compared with group A（6.21 ± 1.73） %, the differences were no significant. Group E（3.49±0.62）%,compared with group A（6.21 ± 1.73）%,group C（6.03±1.43）%], the differences were significant, （all P 〈0.05）. In spleen,group C （7.25± 1.82）% ,group D（8. 63±3.18）%, compared with group A （7.85±2.13）%, the differences were no significant. Group E （3.38 ± 1.37）% compared with group A（7.85±2.13）%,group C （7.25±1.82）%,group D（8.63±3.18）%,the differences were significant,（ P 〈0.05）. Conclusions The ratio of CD4^＋ CD25^＋ T cells to CD4^＋ T cells of asthmatic rat exit predominance expression,which may be one of mechanisms of asthma pathogenesis. Dexamethasone can depress CD4^＋ CD25^＋T cells predominance expression. The changes of CD4^＋ CD25^＋T cells in BALF is consistent with that in the blood and spleen, which suggested that monitoring the changes of CD4^＋ CD25^＋ T cells in blood and spleen can be useful to understand the situatioan of BALF.
出处
《中华哮喘杂志（电子版）》
CAS
2010年第3期146-150,共5页
Chinese Journal of Asthma(Electronic Version)
基金
国家自然基金(30460051)
广西科学基金项目资助(桂科回0342019)
