摘要
以GH和POU1F1作为影响鸡繁殖性状的候选基因,采用PCR-SSCP方法,检测白耳鸡GH外显子4和POU1F1外显子3区域的单核苷酸多态性,并利用最小二乘法分析GH、POU1F1单基因型和两基因聚合基因型与72周龄产蛋数的关联.结果表明,GH基因exon4(C2338G\C2341T)的突变和POU1F1基因exon3的突变(A5331T)基因型CC与白耳鸡的72周龄产蛋数显著相关(P<0.05),基因型AA、CC对产蛋数的加性效应分别为3.80和3.57.聚合基因型AACC个体72周龄产蛋数(除基因型ABCC外)与对照组差异显著(P<0.05).两基因间的互作效应不显著(P>0.05).扩繁F1代聚合基因型AACC个体72周龄产蛋数与前一世代相当.
The chicken GH 和 POU1F1 gene was selected as candidate genes,PCR-SSCP method was used to identify the polymorphism sites in the exon4 region of GH gene and in the exon3 region of POU1F1 gene. Association between the founded polymorphism sites as well as pyramiding genotypes and egg production traits were tested by least square analysis. The results showed the founded polymorphism sites （C2338G／C2341T） of GH gene exon4 had a significant correlation with the egg number at 72 weeks（P0.05）. Additive effects of genotype AA for GH genes were 3.80. The founded polymorphism sites（A5331T） POU1F1 gene exon3 had a significant correlation with egg production at 72 weeks age. Additive effects of genotype CC for POU1F1 genes were 3.57. The pyramiding genotype AACC had higher 72 weeks of age egg production compared to that of other pyramiding genotypes（P0.05）. Its interactive effect was not significant （P0.05）. Eee production of generation F1 was much the same as that of their motherhood.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第4期445-448,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家"863"计划项目(2008AA101009-7)
(2006AA10Z1D8)
国家科技支撑计划项目(2008BADB2B01)
江苏省科技支撑计划项目(BE2008361)
