摘要
以MS为基本培养基,用大蒜茎尖外植体培养的试管苗进行离体培养试验.结果表明,多效唑对大蒜试管鳞茎形成和膨大作用显著,多效唑质量浓度为2～8mg/L时,随着质量浓度的增加,多效唑对鳞茎形成与分化的抑制程度加深,叶片和根系变短、变细弱;诱导鳞茎形成和膨大的最适多效唑质量浓度为6mg/L;多效唑处理后,大蒜试管苗叶鞘中内源激素IAA与ABA的含量比急剧下降,降低幅度越大,鳞茎形成、膨大的效果越好.
In vitro experiments were carried out, using test-tube seedings of garlic stem tip as explants and taking MS as basic culture medium.The results showed that PP333 have a significant effect on formalion of garlic bulb and enlargement in vitro. Within the 2～8 mg/L concentration range, raising concentration could inhibit the formation and differentiation of the bulb, the leaves and roots were shortened and thinned. The 6 mg/L PP333 was the optimal treatment concentration for inducing formation and developmen of bulb. Variations in contents of endogenous bormone indicated that after PP333 treatment, ratio of IAA to ABA inside leaf sheath in the test-tube garlic seedlings dropped suddenly, the greater the decrease, the better the expanding in the formation of bulb.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第4期422-425,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
农业部公益性行业(农业)科研专项(200903018)
关键词
大蒜
多效唑
试管鳞茎
内源激素
garlic
PP333
bulblet in vitro
endogenesis hormones
