摘要
观察榄香烯乳对Ｂ１６黑色素瘤细胞株是否具有诱导分化作用。通过ＭＴＴ显色法测定榄香烯乳在体外对Ｂ１６细胞株的ＩＣ５０，腹腔给药观察榄香烯乳对体内Ｂ１６细胞株皮下、肺转移结节的抑制作用．选取低于ＩＣ５０的药物浓度研究榄香烯乳作用后Ｂ１６细胞株超微结构和成瘤性的改变．榄香烯乳对体内外Ｂ１６细胞株均有抑制作用．药物作用后，瘤细胞生长抑制，增殖减慢，接触抑制恢复，成瘤性降低，电镜观察细胞恶性度下降，出现较为成熟的黑色素颗粒．在低浓度时。
To determine if elemene emulsion induces B16 cells differentiation. The MTT method was used for the determination of IC50 of B16 cells by elemene emulsion. The inhibition of the subcutaneous and lung metastasis tumor in mice was observed by injected intraperitoneally elemene emulsion. we studied the ultrastruture and tumorigenesis of B16 cells in low drug dosage. Both in vitro and vivo B16 cells were inhibited by elemene emulsion. Besides, the ultrastructure showed the morphological changes, such as microvilli decrement, nucleus pyknosis, typical melanogen. Signs of induction of differentiation were observed in low dosage.
出处
《南京大学学报（自然科学版）》
CAS
CSCD
1999年第2期230-235,共6页
Journal of Nanjing University（Natural Science）
