摘要
采用RACE技术从西伯利亚蓼中克隆了锰超氧化物歧化酶基因PsMnSOD的cDNA(GenBank登录号FJ848572)。长为792bp的PsMnSODcDNA序列含有编码234个氨基酸的705bp的开放读码框、57bp的5'非翻译区和30bp的3'非翻译区。构建了PsMnSOD基因的酵母表达载体pYES2-PsMnSOD并将其转化到野生型酵母菌株中。分析PsMnSOD基因在酵母中的表达对酵母SOD酶活性的影响及其对盐胁迫、PEG胁迫、高温和低温胁迫下抗逆性影响的结果表明,PsMnSOD基因在酵母中表达后酵母SOD酶活性提高,酵母对盐渍、PEG、高温和低温等非生物胁迫的抗逆性也增强。
In this research,PsMnSOD cDNA （GenBank No.FJ848572） was isolated from Polygonum sibiricum by RACE technology.The nucleotide sequence of PsMnSOD cDNA is 792 bp containing an open reading frame of 705 bp encoding 234 amino acids,with a 5＇ untranslated region of 57 bp and a 3＇ untranslated region of 30 bp.The PsMnSOD gene yeast expression vector pYES2-PsMnSOD was constructed and transformed into wild yeast strain.The effects of PsMnSOD gene expression on superoxide dismutase activity and resistance to abiotic stress including salt,PEG,high temperature and low temperature of yeast were determined.The results indicated that expression of PsMnSOD gene in yeast improved superoxide dismutase activity and resistance ability to abiotic stress including salt,PEG,high temperature and low temperature.
出处
《植物生理学通讯》
CAS
CSCD
北大核心
2010年第7期653-658,共6页
Plant Physiology Communications
基金
东北林业大学林木遗传育种与生物技术教育部重点实验室开放课题
东北林业大学林木遗传育种黑龙江省重点实验室开放课题
黑龙江省青年科学技术专项资金项目(QC06C044)
关键词
西伯利亚蓼
锰超氧化物岐化酶
抗逆性
Polygonum sibiricum
manganese superoxide dismutase
stress resistance
