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转基因植物选择标记基因删除技术 被引量：8

Technology of Selectable Marker Gene Knockout in Transgenic Plants
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摘要 本文系统地介绍了4种转基因植物选择标记基因删除技术——(共转化法(co-transformation)、转座子法(transposable element)、位点特异性重组法(site-specific recombination)、染色体内同源重组法(intrachromosomal recombination)的基本原理和操作过程,并分析了各自的特点和实际应用情况,比较了它们的优缺点,展望了删除技术的前景。删除技术的发展,无论是对于转基因作物的安全性和商业化,还是转基因技术的发展和完善都有积极的作用。 In this article,we systematically introduced the basic principles and protocols of four methods,co-transformation,transposable element,site-specific recombination,and intrachromosomal recombination,respectively,applied in selectable marker gene knockout in transgenic plants.The prospect of technology of selectable marker gene knockout was offered,through the analysis of their respective features as well as practical applications and the comparison of their advantages and disadvantages.The development of selectable marker gene knockout plays an active role in not only the security and commercialization of transgenic crops,but also the development and improvement of transgenic technology.
作者 魏毅东 许惠滨 张建福 谢华安
机构地区 农业部闽台农作物种质资源利用重点开放实验室 福建省农业科学院水稻研究所
出处 《分子植物育种》 CAS CSCD 2010年第4期804-809,共6页 Molecular Plant Breeding
基金 国家转基因生物新品种培育重大专项(2008ZX08001-006) 福建省农业科学院创新团队项目之一(STIF-Y04)共同资助
关键词 转基因植物 选择标记 基因删除 Transgenic plants Selectable marker Gene knockout
分类号 Q943.2 [生物学—植物学]
  • 相关文献

参考文献28

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二级参考文献28

  • 1[1]Breitler J.C., Meynard D., van Boxtel J., Royer M., Bonno F.,Cambillau L., and Guiderdoni E., 2004, A novel two T-DNA binary vector allows efficient generation of marker-flee transgenic plants in three elite cultvars of rice (Oryza sativa L.), Transgenic Res., 13(3): 271-287
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分子植物育种
2010年 第4期

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