摘要
用Ｍｅｒｉｆｉｅｌｄ固相法合成了荞麦花粉肽ＢＰＰ－１及其类似物ＢＰＰ－１－３，ＢＰＰ－１－４和ＢＰＰ－２。通过ＦＴ－ＩＲ和ＣＤ谱初步讨论了ＢＰＰ－１的二级结构，结果表明其水溶液构象属于无规卷曲和β－折叠，但在三氟乙醇中，ＣＤ谱在２０６ｎｍ和２２２ｎｍ附近呈双负峰形，分子形成了α－螺旋构象。研究了ＢＰＰ－１，ＢＰＰ－１－３和ＢＰＰ－２对动物和人淋巴细胞的增殖应答功能和抑制释放ｓＩＬ－２Ｒ的作用，结果显示它们具有较强的促免疫活性。
Buckwheat pollen peptide (BPP 1) and its three analogues were synthesized using Merrifield solid phase method. The secondary structure of BPP 1 in solution was studied by FT IR and CD spectra. It was considered that the conformation of the peptide was a random coil and β sheet. In TFE, characteristic negative extrema near 222 and 206nm were observed in its CD spectra. It seems that the BPP 1 is in the form of a α helix. The effect of these peptides on spleen lymphocytes from mice and on release of sIL 2R from human lymphocytes were studied. The results showed that these peptides exhibit certain immune activity.
出处
《北京大学学报（自然科学版）》
CAS
CSCD
北大核心
1999年第2期195-200,共6页
Acta Scientiarum Naturalium Universitatis Pekinensis
基金
国家自然科学基金
