摘要
目的
建立七味通痹口服液中青藤碱的含量测定方法 .方法
采用高效液相色谱法（HPLC）对青藤碱进行含量测定,色谱柱为：Waters XTerra
RP18（250×4.6 mm,5μm）：流动相为：乙腈-0.05%三乙胺水溶液（30：70）;检测波长为262
nm. 结果 青藤碱在0.248～2.480 μg范围内线性关系良好,平均加样回收率为98.82%,RSD为0.13%.结论
HPLC法可用于七味通痹口服液的质量控制.
Objective To establish the method for the content determination of sinomenine in Qiwei Tongbi oral liquid. Methods Sinomenine of Qiwei Tongbi oral liquid was determined by HPLC, with the column being Waters XTerra RP18 （250×4.6 mm, 5 μm）; the mobile phase setting as acetonitrile-0.05% triethylamine aqueous solution （30 ： 70）, the column temperature setting at 25℃, flow rate being 1.0 ml/min, and the detective wavelength being 262 nm. Results There was a good linearity of sinomenine in the range of 0.248-2.480 μg, and the average recovery was 98.82% with RSD of 0.13%. Conclusion This method was simple, accurate, reproducible, and thus could be used for quality control of Qiwei Tongbi oral liquid.
出处
《国际中医中药杂志》
2010年第4期351-352,共2页
International Journal of Traditional Chinese Medicine
基金
基金项目：国家科技部重大新药创制（项目编号：2009zx09313-032）
