摘要
目的建立盐酸青藤碱缓释微丸的含量测定方法。方法Diamonsil—C18柱，流动相为乙腈-0.78％（ρ）磷酸二氢钠溶液（体积比12：88），检测波长为265nm，柱温为30℃，流速为1．0mL／min。结果盐酸青藤碱在20～100μg／mL内呈良好的线性相关性，r=0．9999，方法精密度RSD为0．4％（H=6），平均回收率分别为99．7％，RSD为0．6％（H=9）。结论该方法简便、准确、快速，可用于盐酸青藤碱缓释微丸的含量测定及质量控制。
Objective To establish an HPLC method for determining the content of sinomenine hydrochloride in sustained-release pellets. Method The sample was analyzed on a Diamonsil C18 （4.6 mm ×200 mm, 5μm ） column using acetonitrile and 0.78% （ρ） NaH2PO4 solution （12： 88） as mobile phase. The detection wavelength was 265 nm and flow rate 1.0 mL/min, Results The linear range of sinomenine hydrochloride was 20 - 100 μg/mL （ r =0. 999 9）. The average recovery was 99.7% and RSD 0. 6%, Conclusion This method is simple, rapid and accurate, which is suitable for the quality control of sinomenine hydrochloride sustained-release pellets.
出处
《广东药学院学报》
CAS
2007年第2期164-165,185,共3页
Academic Journal of Guangdong College of Pharmacy
关键词
盐酸青藤碱
微丸
高效液相色谱法
sinomenine hydrochlofide
sustained - release pellets
HPLC
