摘要
目的探讨CD40基因启动子区-1C/T单核苷酸多态性位点(SNP)与冠状动脉(冠脉)不稳定粥样斑块的相关性。方法 474例冠心病(CHD)患者冠脉造影(CAG)粥样斑块形态学分型为Ⅰ型(131例)、Ⅱ型(179例)和Ⅲ型(164例),采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测CD40基因多态性及测序鉴定;并与225例CAG正常者对照。结果 C等位基因频率CHD组明显高于对照组(52.6%vs.42.8%)(P<0.05),而T等位基因频率CHD组明显低于对照组(20.3%vs.32.0%)(P<0.05);CHDⅠ、Ⅱ、Ⅲ亚组C等位基因频率分别为43.1%、56.4%、56.1%,T等位基因频率分别为56.9%、43.6%、43.9%,Ⅱ型、Ⅲ型亚组C等位基因频率高于Ⅰ型亚组,而T等位基因频率低于Ⅰ型亚组(P<0.05)。结论 CD40-1C/TSNP位点与冠脉斑块的不稳定性相关,C等位基因增加了斑块的易损风险。
Objective To investigate whether CD40 gene （-1C/T） single nucleotide polymorphism（SNP） is associated with unstable coronary atherosclerotic plaque.Methods According to the morphological division of the plaque,474 patients with coronary angiography were divided into three groups of type Ⅰ lesion（131 cases）,typeⅡ lesion （179 cases） and type Ⅲ lesion（164 cases）.The results were compared to those with normal CAG（225 cases）.The gene polymorphism was measured by the polymerase chain reaction and restriction fragment length polymorphism （PCR-RFLP） and identified by sequencing.Results C allele frequency in patients with CHD was significantly higher than that in control group（52.6% vs. 42.8%）（P0.05）,while T allele frequency was lower than that in control group（20.3% vs. 32.0%）（P0.05）.Among the subgroups in patients with CHD,the frequencies of C allele were 43.1%,56.4% and 56.1%,and those of T allele were 56.9%,43.6% and 43.9%,respectively.C allele frequencies of typeⅡ and type Ⅲ lesion subgroups were higher than those of type I lesion subgroup（P0.05）.Conclusion CD40-1C/T polymorphism is associated with unstable coronary atherosclerotic plaque.The C allele frequency increases the risk of rupture of vulnerable plaque.
出处
《江苏医药》
CAS
CSCD
北大核心
2010年第13期1507-1510,共4页
Jiangsu Medical Journal
基金
江苏省医学重点人才基金(RC2007033)
镇江市社会发展基金(SH2008030)
关键词
冠心病
单核苷酸多态性
CD40
Coronary heart disease
Single nucleotide polymorphism
CD40
