摘要
目的:研究补肾益气方对皮质酮致损伤大鼠胸腺细胞周期和p16、CDK4、IL-2Rα表达的影响。方法:1×10-5M皮质酮处理新生乳鼠胸腺细胞。PI染色结合流式细胞术分析细胞周期分布;Realtime PCR和Western blotting法检测p16、CDK4、IL-2Rα的表达变化。结果:胸腺细胞经皮质酮处理后,显著阻滞于G1期;p16表达显著升高,CDK4、IL-2Rα表达降低(P<0.05)。与皮质酮组比较,补肾益气组G1期细胞数量减少,S期细胞数量升高(P<0.01);p16基因表达降低,IL-2Rα表达升高;CDK4表达无明显变化。结论:补肾益气方能抑制p16的表达,缓解皮质酮对胸腺细胞周期的抑制作用,提高胸腺依赖性免疫功能。
Objective：To investigate the mechanism of Bushenyiqi（补肾益气） Formula （BSYQ） on the cell cycle and immuno function in corticosterone damageed rat thymocytes.Methods：Prepared the BSYQ contained rat serum.Thymocytes were divided into 3 groups：（A） control group;（B）model group;（C） BSYQ group.Cell cycle was measured by PI staining and FCM.The expression of p16,cdk4,IL-2Rα were measured by Real-time PCR and Western Blotting.Results：10-5 M corticosterone arrested cell cycle at G1 phase,up-regulated the expression of p16,down-regulated the expression of CDK4 and IL-2 Rα.Comparing with model group,BSYQ group showed higher expression of IL-2 Rαand lower expression of p16 （P0.05）,the number of cells in G1 phase decreased （P0.05）.Conclusion：BSYQ can postpone the immune functional deterioration induced by corticosterone.The possible mechanism is to regulate the expression of p16,IL-2 Rα.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2010年第3期41-44,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然基金(30873317)
上海市教育委员会重点学科建设项目(J50301)
上海市教委基金(No.06cz011)
