摘要
目的:探讨被动吸烟人群尼古丁受体3基因(CHRNA3)启动子区遗传变异与肺癌发病的关系。方法:用病例-对照的研究方法,收集250例被动吸烟原发性肺癌患者和250例性别相同、年龄相差±1岁被动吸烟正常对照,采用PCR-RFLP技术检测CHRNA3基因启动子单核苷酸多态位点rs6495309(T>C)的基因型,用SAS 9.13软件进行非条件Logistic回归校正混杂因素的影响,分析基因变异与肺癌发病的关联。结果:基因型频率分析均符合Hardy-Weinberg平衡,P=0.109 6;CHRNA3 rs6495309(T>C)位点变异在肺癌与对照中的分布差异有统计学意义,P=0.043 2;携带CC基因型个体发生肺癌的危险性较TT基因型个体增加81%(95%CI=1.07～3.06;P=0.019);rs6495309C变异基因型(rs6495309TC和rs6495309CC)患肺癌的危险性增加(adjusted OR=1.63;95%CI=1.06～2.50;P=0.025)。发现被动吸烟来自于父母人群时该位点变异在肺癌与对照中分布差异有统计学意义,P=0.047 9。结论:CHRNA3基因启动子rs6495309(T>C)变异的被动吸烟人群肺癌发病危险性较高,且其危害性主要表现为被动吸烟来源于父母的人群。
OBJECTIVE： To investigate the association between the genetic variant in CHRNA3 promoter and lung cancer risk in passive smoking population.Methods： A case-control study of 250 lung cancer cases and 250 age-and sex frequency-matched cancer-free passive smoking controls were performed in a Chinese population to investigate the risk of lung cancer for common polymorphism rs6495309（TC）.CHRNA3 genotype was determined by PCR-RFLP analysis and SAS 9.13 was used to analysis the genetic variant and lung cancer risk through non-conditional logistic regression.RESULTS： The observed genotype frequency of rs6495309（TC） was in agreement with the Hardy-Weinberg equilibrium in the control subjects（P=0.109 6） and was significantly difference among case and control（P=0.043 2）.Compared with the TT genotypes,the association between the CC homozygote and the risk of lung cancer was statistically significant（adjusted OR=1.81;95%CI=1.07-3.06;P=0.019）.The carriers of variant（risk） alleles（rs6495309C：rs6495309TC and rs6495309CC） were associated with significantly increased risk of lung cancer（adjusted OR=1.63;95%CI=1.06-2.50;P=0.025）;The source of passive smoking were analyzed and the observed genotype frequency of rs6495309（TC） was only significantly difference among case and control whose passive smoking was from parents（P=0.047 9）.CONCLUSION： The CHRNA3 promoter variant rs6495309（TC） can significantly increase the risk of lung cancer in passive smoking population,especially in the population whose passive smoking was from parents.
出处
《中华肿瘤防治杂志》
CAS
2010年第13期972-975,990,共5页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金项目(30872142
30872178)
广东省自然基金资助(070055
070060
8251018201000005)
