摘要
探讨使用硅胶柱层析法从杜仲粕中分离纯化桃叶珊瑚苷(Au),优化柱层析吸脱附条件,用薄层色谱法(TLC)和高效液相色谱法(HPLC)对产品进行定性定量分析。结果表明:优化的硅胶用量为生药质量的2倍,使用V(甲醇):V(氯仿):V(石油醚):V(乙酸乙酯)为7:1:0.5:1.5的混合溶剂在1.0mL/min流速下进行洗脱,通过收集含Au高的组分,经减压浓缩、结晶、过滤、重结晶等过程,可获纯度为96.56%的桃叶珊瑚苷产品。
A silica gel chromatographic method was presented for the purification of aucubin （Au） from the crude extract of the residue of Eucommia ulmoides seed meal left after linolenic acid extraction.Subsequently,the extracted product was analyzed qualitatively and quantitatively.An optimal purification of Au was achieved by using a 2-fold mass of silica gel for Au adsorption followed by desorption with a 70% methanol/chloroform/petroleum ether/ethyl acetate mixture （7：1：0.5：1.5,V/V） at a flow rate of 1.0 mL/min.The eluate fractions were harvested and subjected to vacuum condensation,crystallization,filtration and recrystallization,and a final Au product with 96.56% purity was obtained.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第14期58-61,共4页
Food Science
基金
湖南省教育厅优秀青年项目(B060701)
关键词
桃叶珊瑚苷
柱层析
硅胶
纯化
aucubin
column chromatography
silica gel
purification
