摘要
目的：应用树鼯进行Oltipraz（OLT）对黄曲霉毒素B1（AFB1）的化学预防研究。方法：实验树鼯按不同处理方法分为4组：A组：正常对照二组：AFB1；C组：AFB1十药1（OLT7次／周，共5周）；D组：AFB1＋药2（OLT1次／周，共5周）。动物处死后肝组织作低温石蜡连续切片，分别染γ－GT、GST－P及HE。结果：C组与A组树鼯肝内均有较多沿汇管区分布的γ－GT阳性肝细胞群；而D组与B组肝内γ－GT阳性肝细胞群量明显减少（P＜0．01）。结论：实验结果提示了OLT具有对抗AFB1毒性的化学预防作用，并且此种作用具有一定的剂量－效应关系。本研究结果为进一步将树鼯应用于类似短期实验研究打下基础。
Objective:To study the protective effect of OLT(oltipraz) on AFB1(aflatoxin B1) by using the tree shrews,a kind of small lowest primate.Methods:The animals were divided into 4 groups:A:normal control; B: AFB1 only; C:AFB1+OLT daily; D: AFB1+OLT weekly. All of the animals were killed at the end of the nine-week experiment. The slides of the liver tissues were stained for γ-GT, GST-P and HE respectively. Results: The extents of the γ-GT positive liver cell group, which appeared mostly around the periportal areas, were similar in the group A and C. On the contrary, the γ-GT positive liver cell groups were significantly reduced in group B and D(P<0.01). Besides, the liver cell necrosis showed by HE staining was more obvious in group B and D than in group A and C. Conclusion: OLT not only has the chemoprotective effect on AFB1, but this effect is dose-relaed as well. The result of this study provides the foundation for the similar study by using tree shrews in the future.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1999年第1期34-36,共3页
Chinese Journal of Cancer
基金
美国JohnsHopkins大学T.Kensler教授资助
