摘要
目的：对广东地区37例肝癌染色体9p的9个位点进行微卫星多态性分析，明确这些位点染色体等位基因杂合性丢失（LOH）的情况，定位该区域可能存在的与肝癌有关的肿瘤抑制基因。方法：对所取位点用PCR法进行微卫星多态性分析并确定发生LOH的位点；结果：30例（80.80％）在至少一个位点发生LOH，其中LOH频率最高的位点是D9554（60．60％），LOH频率高于50％的位点有位于9p21带的1FNA，D9S1747和D9S1752，6例在所有能够提供信息的位点均发生LOH，7例在所有9个位点均未发生LOH。提示本地区肝癌染色体9P缺失的区域主要位于D9554（9p24），和DgS1752，D9S1747，1FNA（9p21）。结论：本研究首次发现肝癌在染色体9p24区发生高频率LOH，染色体9p21区也发生高频率LOH，提示在染色体9p24和9p21区域可能存在多个与肝癌相关的肿瘤抑制基困。
篜urpose: We analyzed the microsatellite instability of 9 loci on chromosome 9p in 37 hepatocellular carcinomas (HCC) from Guangdong area to detect the loss of heterozygosity (LOH) on these loci,in order to find the possible tumorsuppressor gene which associated with HCC. Method: LOH on the 9 loci were ensured by PCR based microsatellite instability analysis. Result:In 37 cases of HCC, at least 30 cases have LOH occurred of non-informative at one locus, 6 of the 30 cases HCC occurred LOH at all the nine loci on chromosome 9p. High frequent LOH (>50%) occurred at four loci including D9S54 (60. 60% ), D9S1752 (53. 84% ), IFNA (52.17% ) and D9S1747 (50.00%). Seven cases have no LOH occurred of non-informative at all the nine loci. The results indicated that high frequent LOH occurred at chromosome 9p24 region and at chromosome 9p21 region. Conclustion: From our investigation, we firstly found that high frequency of LOH occurred at 9p24 band, and higher frequentcy of LOH were also occurred at 9p21 region. These results indicate that more than one TSGs related to HCC may harbored at chromosome 9p24 region and 9p21 region.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1999年第1期16-19,共4页
Chinese Journal of Cancer
关键词
肝肿瘤
肝细胞癌
杂合性丢失
等位基因
Hepatocellular cell carcinoma
Loss of heterozygosity
Polymerase chain reaction
Tumor suppressor gene
