摘要
目的 寻找胶质母细胞瘤（ＧＢＭ）９号染色体上可能存在肿瘤抑制基因的杂合性丢失（ＬＯＨ）区域，为发现和定位肿瘤抑制基因（ＴＳＧ）提供线索和依据。方法 应用聚合酶链反应（ＰＣＲ）方法，采用荧光标记引物和３７７型ＤＮＡ序列自动分析仪，分析２１例 ＧＢＭ ９号染色体上 ２０个微卫星多态性标记的ＬＯＨ。结果 ２１例 ＧＢＭ中，在１４例的９号染色体上检测到ＬＯＨ，３３．０％（９３／２８）可提供信息位点存在ＬＯＨ。在９ｐ和９ｑ上存在ＬＯＨ的ＧＢＭ分别有１４例和１０例。在位于９ｐ２１－２３的Ｄ９Ｓ２８６位电以及９ｐ２１的Ｄ９５２８５ｓ～Ｄ９Ｓ１５７位点间区域检测到较高ＬＯＨ率，分别为５２．６％、４３．８％～４６．７％．结论 染色体９ｐ上等位基因的丢失可能在ＧＢＭ分子水平发病机制中起着重要作用。在９ｐ２１－２３的Ｄ９Ｓ２８６位点、９ｐ２１的Ｄ９Ｓ２８５～Ｄ９Ｓ１５７位点间区域可能存在与ＧＢＭ相关的多个ＴＳＧ，可能包括位于９ｐ２１上的ｐ１６和ｐ１５基因，在ｐｌ６、ｐ１５所在染色体区域的远端９ｐ２１－２３可能存在其他未知的ＴＳＧ。
Objective To locate the deletion regions possibly harboring tumor suppressor genes on chromosome 9 with a view to facilitating the identification of novel tumor suppressor genes in glioblastoma(GMB) .Methods 20 loci on chromosome 9 were examined to detect loss of heterozygosity(LOH) in 21 cases of glioblastoma by PCR based microsatellite polymorphism analy-ses, in which fluorescence-labeled primers and Perkin Elmer 377 DNA Sequencer were applied. Results 66.7%(14/21) infor-mative cases of GBM displayed LOH on chromosome 9, 33.0%(93/282) of informative loci showed LOH in our series, in which the most frequent LOH was observed at locus D9S286(52. 6% ) on 9p21-23 and in the chromosomal region from locus D9S285(43.8%) to D9S157(46.7%) on 9p21. 66.7% of informative cases displayed LOH on 9p and 47.6% on 9q. Conclusions Loss of genetic material on chromosome 9p may play an important role in the molecular genetic pathogenesis of GBM. The chromo-somal regions at D9S286 on 9p21-23 and from D9S285 to D9S157 on 9p21 may harbor several tumor suppressor genes associated with GBM. Besides the well-known TSGs p16 and p15, a novel TSG may be present on the region distal to p16 and p15.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2001年第3期173-176,共4页
Chinese Journal of Nervous and Mental Diseases
