摘要
目的：为了对流感病毒甲型和乙型、副流感病毒１、２和３型、呼吸道合胞病毒及腺病毒３、７型主要呼吸道病毒进行快诊。方法：从１９８６到１９９７年应用杂交瘤单抗技术，制备出以上４类８种抗病毒单抗细胞库，连同用于连接物的抗碱性磷酸酶单抗细胞株，共有１１７株单抗细胞株。将以上４类８种病毒单抗连接抗碱性磷酸酶单抗复合物，以兔抗鼠搭桥进行ＡＰＡＡＰ桥联快速诊断。结果：获得满意结果，此技术结果清晰，无内源性酶干扰，只需１台普通光学显微镜，２～３ｈ可获得准确结果。本系列快速诊断试剂已获得国家４项新药证书及５项生产文号。结论：此抗主要呼吸道病毒单抗细胞库，不仅可提供快诊试剂，也可提供病毒亚型分析。
Objective:The 8 types, 4 kinds of main respiratory viruses which cause human respiratory
diseases, include influenza A、B, parainfluenza virus 1、2、3, respiratory syncytial virus
and adenovirus 3、7.Methods:Had developed a kit to rapidly diagnose these viruses from
1986 to 1997. By the technique of hybridoma, had prepared 117 hybridoma cell strains
secreting McAbs of these 8 types, 4 kinds of viruses and of alkaline phosphatase. The McAbs
form a junctional complex plus alkaline phosphatase anti alkaline phosphatase (APAAP)
bridged rapid diagnosis. Results:The result of the kit is credible, and no disturbance of
Pendogenous enzyme. Performing the diagnosis, only need a general optical microscope and
can finish in 2 or 3 h. The hybridoma cell bank can provide not only the reagents of the rapid
diagnosis, but also the reagents for analysis of virus subgroups, virus variation and gene
expression.Conclusion:The serial reagents of the rapid diagnosis have been granted 4 new
drug certificates and 5 production certificates by the ministry of Health Department of National
Health.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1999年第2期74-76,共3页
Chinese Journal of Immunology
关键词
呼吸道病毒
单克隆抗体
杂交瘤细胞库
Main respiratory viruses Monoclonal antibody Hybridoma cell bank
