摘要
背景:神经干细胞不仅在大脑、脊髓及周围神经的临床应用有大量研究,在内耳的应用也日益受到重视。研究发现利用神经干细胞移植修复内耳毛细胞、螺旋神经节以及听神经损伤是可行的。目的:复习相关文献,就神经干细胞的特性、培养、内耳听力损伤的应用等方面进行综述。方法:由第一作者检索1990/2008PubMed数据库(http://www.ncbi.nlm.nih.gov/PubMed)及万方数据库(http://www.wanfangdata.com.cn)有关神经干细胞培养、分化及在内耳应用等方面的文章。英文检索词为"neuralstemcells,transplant,innerear,peripheralnerve",中文检索词为"神经干细胞,移植,内耳,周围神经"。检索文献量总计107篇,排除重复性研究,初检得到107篇文献,阅读标题和摘要进行初筛,共保留其中的36篇归纳总结。结果与结论:虽然对神经干细胞的生物学特性有了进一步的了解,对神经干细胞的研究取得了重大的进步,在听觉研究中可以应用干细胞移植,再生或保护毛细胞和与之相连的螺旋神经元,给内耳疾病治疗带来广泛的前景;但实现毛细胞再生和重建听觉通路依然是充满希望的挑战。随着神经干细胞基础理论的不断更新和发展,其在临床的应用一定会取得突破性进展。
BACKGROUND：There are numerous studies concerning the clinical application of neural stem cells（NSCs） to the brain,spinal cord and peripheral nerves,and its application to internal ear also has been paid great attention.It is feasible to utilize NSCs transplantation for repair of damages to internal ear hair cells,spiral ganglion and auditory nerve.OBJECTIVE：To review characteristics,culture of NSCs and its application to damage to auditory nerve in the internal ear.METHODS：The first author retrieved PubMed Database（http：//www.ncbi.nlm.nih.gov/PubMed） and Wanfang Database（http：//www.wanfangdata.com.cn） published from 1990 to 2008 for articles addressing culture and differentiation of NSCs,and its application in the internal ear.The key words were ＂neural stem cells,transplant,inner ear,peripheral nerve＂.Repetitive studies were excluded,and 107 articles were primarily collected.Following reading titles and abstracts,a total 36 articles were used for review. RESULTS AND CONCLUSION：Biological features of NSCs are understood,but the studies concerning NSCs have obtained great progress.Stem cell transplantation can be used in the study of auditory sensation.Regeneration or protection of hair cells and connected spiral neurons provide extensive prospects for treating internal ear disease.However,it is a challenge to realize hair cell regeneration and reconstruction of auditory pathway.With the renewal and development of NSCs basic theory,NSCs application to clinic would obtain breakthrough.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第27期5085-5089,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
郑州大学211工程资助项目~~
