摘要
目的探讨缺氧缺血(HI)新生大鼠脑组织超氧化物歧化酶(SOD)和基质金属蛋白酶-3(MMP-3)的表达变化及孕酮对SOD、MMP-3表达的影响。方法新生SD大鼠72只随机分成4组:正常对照组(正常组)、假手术组、HI组和孕酮组,每组18只。建立新生大鼠缺氧缺血性脑损伤(HIBD)模型。电镜观察HIBD新生大鼠血脑屏障的通透性改变。羟胺氧化法检测各组新生大鼠脑组织SOD活性,免疫组织化学法检测各组新生大鼠大脑皮质MMP-3表达。应用SPSS12.0软件进行统计学分析。结果电镜显示正常组和假手术组新生大鼠血脑屏障微血管内皮完整光滑,结构清晰;HI组新生大鼠血脑屏障完整性明显破坏;孕酮组新生大鼠血脑屏障微血管内皮完整性轻微破坏。HI组大鼠脑组织SOD活性较假手术组显著降低(P<0.01),孕酮组新生大鼠脑组织中SOD活性较HI组显著升高(P<0.01)。HI组新生大鼠大脑皮质MMP-3阳性表达较正常组增多,而孕酮组新生大鼠大脑皮质MMP-3阳性表达较HI组减少。结论孕酮可通过减少MMP-3的表达,降低血脑屏障损伤,这可能是其发挥脑保护作用的机制之一。
Objective To explore the expressions of superoxide dismutase （SOD） and matrix metalloproteinase - 3 （ MMP - 3 ） in the brain tissues of newborn rat after hypoxic - ischemic（HI） and the effects of progesterone（PROG） on SOD and MMP - 3 expression. Methods Seventy -two neonatal SD rats were randomly divided into 4 groups：normal control group （normal group）, sham -operated group, HI group and PROG group. The model of neonatal rat with hypoxic - ischemie brain damage（HIBD） was made. The permeability of the blood - brain barrier was observed by using transmission electron microscopy,the level of SOD in the brain was assessed by hydroxylammoniumehloride autoxidation, MMP -3 protein expression in cerebral cortex part of the brain was detected by immunohlstochemical. SPSS 12.0 software was used to analyze the data. Results In normal group and sham - operated group, capillary vessel＇s morphology of blood brain barrier of neonatal rats were integrity and smooth, and structure were clarity. The permeability of blood - brain barrier in HI group was obviously enhanced and had slight changes in PROG group. Compared with the sham - operated group,the level of SOD in HI group significantly decreased （P 〈 0.01 ） ; and compared with HI group,the level of SOD in PROG group significantly increased（P 〈0.01 ）. The positive expression of MMP - 3 in HI group was significantly increased compared with that in normal group,and the PROG group was decreased compared with that in HI group. Conclusion By reducing MMP - 3 expression,PROG reduces the blood - brain barrier damage,which may exert one of its protective mechanisms in the protection of the brain.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2010年第14期1065-1067,共3页
Journal of Applied Clinical Pediatrics
基金
河南省科技攻关重大项目(092101310600)
关键词
孕酮
缺氧缺血性脑损伤
血脑屏障
基质金属蛋白酶-3
超氧化物歧化酶
progesterone
hypoxic - ischemie brain damage
blood brain battier
matrix metalloproteinase - 3
superoxide dismutase
