摘要
目的:探讨外源性孕激素黄体酮对自由落体脑损伤模型大鼠大脑皮质损伤后周围水肿及神经细胞凋亡的治疗作用。方法:实验于2004/2005在南方医科大学珠江医院全军神经医学研究所进行,取60只雌性SD大鼠,行双侧卵巢切除术,送回鼠笼饲养10d后随机分为5组,每组12只:①正常组,不作任何处理。②假损伤组,仅在左顶叶损伤部位处去除颅骨切除术,不给药。③损伤组,自由落体(1000g·cm)打击左顶叶制成脑损伤模型,不给药。④损伤+生理盐水组,同前制成脑损伤模型,术后每12h腹腔注射生理盐水2mL,连续3d。⑤损伤+黄体酮组,制成脑损伤模型,术后每12h腹腔注射黄体酮20mg/kg,连续3d。术后第4天早上处死大鼠,观察脑组织含水量,用苏木精-伊红染色观察脑组织病理形态学变化,Hoechst染色观察神经细胞凋亡的情况。结果:60只大鼠全部进入结果分析。①脑组织病理形态学变化:苏木精-伊红染色显示损伤组和损伤+生理盐水组表现为受损侧和对侧均有严重脑水肿,血管渗出增加、神经细胞坏死,而损伤+黄体酮组则明显减轻。②脑组织含水量:损伤组和损伤+生理盐水组均显著高于损伤+黄体酮组犤(82.5±1.21)%,(82.6±1.03)%,(80.8±0.78)%,P<0.01犦。③Hoechst染色细胞凋亡计数:损伤组和损伤+生理盐水组显著高于损伤+黄体酮组犤(31.50±2.65),(28.
AIM: To study the effect of ectogenesis progestogen progesterone on peripheral edema and neurocyte apoptosis in rats following cerebral cortex injury induced by free fall.METHODS: The experiment was performed in the Institute of Neurology, Zhujiang Hospital of Southern Medical University from 2004 to 2005.A total of 60 male SD rats fed for 10 days after bilateral oophorectomy were assigned to receive no treatment (control group), craniectomy in left parietal lobe and without drug (sham injury group), free fall injury in left parietal lobe and without drug (injury group), free fall injury in left parietal lobe plus 2 mL saline for 3 days 12 hours after operation (injury plus saline group), or free fall injury in left parietal lobe plus 20 mg/kg progesterone for 3 days 12 hours after operation (injury plus progesterone group), 12 rats in each group. At the fourth morning, rats were killed to observe the water content of brain; changes of pathomorphology in brain tissue and neurocyte apoptosis were observed by using hematoxylin and eosin staining and Hoechst dyeing.RESULTS: All 60 rats were involved in the result analysis.① Hematoxylin and eosin staining indicated serious cerebral edema, increased blood vessel exudation and neurocyte necrosis were found in injuried side and uninjuried side in both injury group and injury plus saline group, but it was relieved in injury plus progesterone group. ② The water content in injury group and injury plus saline group was higher than that in injury plus progesterone group[(82.5± 1.21)% , (82.6± 1.03)% , (80.8± 0.78)% ,P< 0.01]; ③ Hoechst dyeing showed that the number of apoptosis cells in injury group and injury plus saline group was higher than that in injury plus progesterone group(31.50± 2.65,28.75± 2.22, 11.00± 1.41, PCONCLUSION: Progesterone can obviously decrease the permeability of blood brain barrier, alleviate the size and degree of edema and blood vessel exudation, and reduce the infiltration of neutrophilic granulocyte and neurocyte necrosis after brain injury.
出处
《中国临床康复》
CSCD
北大核心
2005年第17期70-71,i004,共3页
Chinese Journal of Clinical Rehabilitation
基金
广州医学院第一附属医院科研基金项目~~
