摘要
目的研究穿心莲内酯对巨噬细胞环氧化酶2(COX-2)表达及其主要产物前列腺素E2(PGE2)生成的影响。方法取生长良好的小鼠巨噬细胞RAW264.7,加入不同浓度的穿心莲内酯(终浓度1、10、50μmol/L)进行预干预,1h后再加入脂多糖(LPS,终浓度1μg/mL)刺激,并设空白组和穿心莲内酯单独作用组作为对照组。取培养18h细胞上清,用Elisa法检测PGE2生成量;取培养24h细胞,提取总蛋白,用WesternBlot法检测穿心莲内酯对COX-2蛋白表达的影响。结果 LPS可以显著诱导RAW264.7细胞COX-2表达和PGE2的生成,与对照组比较P<0.01;穿心莲内酯预干预可以抑制LPS诱导的COX-2蛋白表达,下调PGE2的生成。结论穿心莲内酯可通过降低LPS诱导的巨噬细胞COX-2表达和PGE2生成,发挥抗炎作用,这可能是其抗炎的作用机制之一。
Objective To investigate the inhibit effects of andrographolide on LPS-induced cyclooxygenase-2 （COX-2） expression and prostaglandins E2 （PGE2） synthesis in macrophage cells.Methods A macrophage cell line RAW264.7 cells were cultured and pretreated with different concentration of andrographolide （final concentration 1,10,50μmol/L） for 1 hours.Then the cells were stimulated with lipopolysaccharide （LPS,1μg/mL） and incubated at 37℃.The production of PGE2 in the supernatant was measured by Elisa assay.The expression of COX-2 protein were determined by Western Blot.Results After stimulated with LPS,the content of PGE2 and the expression of COX-2 were higher than those in the control group.Andrographolide signi？cantly inhibited LPS-induced PGE2 production in a concentration-dependent manner.Andrographolide also inhibited LPS-induced COX-2 expression in protein level.Conclusion These results indicated that andrographolide might effectively blocks acute production of PGE2 and,at the same time,inhibits expression of COX-2 in LPS-induced macrophage cells.It could be speculated that anti-inflammation damage by andrographolide might contribute,in part at least,to the suppression of COX-2 expression and PGE2 synthesis.
出处
《中国医药指南》
2010年第22期5-6,共2页
Guide of China Medicine
基金
国家自然科学基金(30901980)
关键词
穿心莲内酯
巨噬细胞
环氧化酶2
前列腺素E2
内毒素
Andrpgrapholide
Macrophage cell
Cyclooxygenase-2
Prostaglandins E2
Lipopolysaccharide
