摘要
目的：探讨一氧化氮、内皮素和循环内皮细胞在支气管哮喘中的作用。方法：哮喘患儿６１例，４０名健康儿童为对照，测定其血浆ＮＯ－２／ＮＯ－３、ＥＴ、ｃＧＭＰ和循环内皮细胞（ＣＥＣ）水平，用过敏性哮喘豚鼠进行验证。结果：哮喘患儿血浆ＮＯ－２／ＮＯ－３、ｃＧＭＰ和ＣＥＣ水平均明显升高（Ｐ＜０．０５～００１），其中重度哮喘患儿血浆ＮＯ－２／ＮＯ－３和ＣＥＣ水平较轻中度哮喘患儿更高（Ｐ均＜００１）；重度哮喘患儿血浆ＥＴ水平明显升高（Ｐ＜００１）；哮喘患儿血浆ＮＯ－２／ＮＯ－３与ｃＧＭＰ和ＣＥＣ水平呈显著正相关（Ｐ＜００５～００１）。过敏性哮喘豚鼠ＢＡＬＦ中ＮＯ－２／ＮＯ－３、ＥＴ及ｃＧＭＰ水平均明显升高（Ｐ均＜００１）；血浆中ｃＧＭＰ和ＣＥＣ水平亦升高（Ｐ均＜００５）；糖皮质激素可降低ＮＯ－２／ＮＯ－３、ＥＴ、ｃＧＭＰ和ＣＥＣ水平。结论：哮喘时ＮＯ－２／ＮＯ－３、ＥＴ和ＣＥＣ产生过多，它们在哮喘发病机制中占有重要地位，而且血浆ＥＴ和ＣＥＣ水平可能与哮喘病情相关。
AIM:To investigate the roles of NO,endothelin(ET) and circulating endothelial cells (CEC) in the pathogenesis of asthma. METHODS:Plasma nitrite and nitrate (NO - 2/NO - 3), ET, cGMP and CEC levels in 61 asthmatic children and 40 matched normal children were examined. In addition, the plasma and bronchoalveolar lavege fluid (BALF) NO - 2/NO - 3, ET, cGMP and CEC levels in guinea-pigs with allergic asthma were determined.RESULTS:Asthmatic children had increased amounts of NO - 2/NO - 3, cGMP and CEC compared with normal control subjects ( P<0.05, P<0.01 ).Among them, 15 severe asthmatic children had significantly higher levels of ET than normal subjects ( P<0.01 ). Elevations of NO - 2/NO - 3 were correlated with cGMP and CEC( P <0.05, P< 0.01). Guinea-pigs with allergic asthma had increased amounts of NO - 2/NO - 3,ET and cGMP in BALF than normal control subjects ( P<0.01 ),so did cGMP and CEC in plasms( P <0.05). Glucocorticoid may have reduced NO - 2/NO - 3, ET, cGMP and CEC contents and even have therapeutic effects.CONCLUSIONS:There were increased production of NO - 2/NO - 3, ET, and CEC in asthma, they may play an important roles in the pathogenesis of asthma. ET and CEC may be useful in forecasting the severity of illness.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1999年第4期373-375,共3页
Chinese Journal of Pathophysiology
关键词
哮喘
一氧化氮
内皮素
循环内皮细胞
MeSH Asthma
Nitric oxide
Endothelins
Endothelium
Cells
Child
Guinea-pigs
