摘要
改进左向右分流血管吻合方法，建立动力型肺动脉高压模型。方法选择本地杂种犬６条，２条利用左肺动脉上叶分支搭桥与降主动脉行端侧吻合（人工ＰＤＡ），４条犬利用左锁骨下动脉与左肺动脉行端侧吻合（Ｂｌａｌｏｃｋ法），吻合口４～６ｍｍ，术后笼中饲养４～６个月。通过三腔漂浮导管测定平均肺动脉压（ＭＰＡＰ）、肺毛细血管楔嵌压（ＰＣＷＰ），计算肺血管阻力（ＰＶＲ），取心脏测定右心室（ＲＶ）与左室加室间隔（ＲＶ＋Ｓ）重量，肺组织病理切片观察内膜、中膜变化，测定中膜厚度百分比（ＭＴ％）和肺小血管肌性动脉百分比（ＭＡ％）。结果ＭＰＡＰ和ＰＶＲ明显升高，右心室肥厚，肺小血管内膜增生，中膜增厚。结论本实验未环缩对测肺动脉，形成了全肺ＰＨ，符合疾病的病理生理改变，而且操作比较简便，模型可靠，重复性好，适合研究选用。
Objective To build a novel animal model of hyperkinetic pulmonary hypertension by improving on the vascular anastomosis of left-to-right shunt. Methods End-to-side anastomoses of the upper branch of left pulmonary artery and descending aorta were performed in 2 canines, and between leftsubclavian artery and left pulmonary artery were done in 4 canines. Mean pulmonary artery pressure(MPAP) and pulmonary capillary wedge pressure (PCWP) were measured by Swan Ganz, Cardiac output(CO) was valued with resistance method after 4-6 months follwing shunt, and pulmonary vascular resistance (PVR) was caculated. Aftersacrificed, the weight of right ventricle and left ventricle with septumwas weighed. Furthermore medial thickening of intra-acine pulmonary arteries (IAPA) were analysed byLABS image system and muscular arteries in numbers were counted under light microscope. ResultsCompared with pre-shunt, MPAP and PVR elevated obviously, Correlated with controls, right ventricledemonstrated hypertrophy, medial thickening of IAPA enlarged, and muscular arteries in numbers increased. Conclusions The animal model of pulmonary hypertension developed in our study totally imitatesthe status of pulmonary hypertension pathophysiology without stenosed opposite main pulmonary arteryand/or ligating some branchs of isolateral pulmonary artery. Besides, this model succeeded in its relativelysimple, reliable and stable, can be provided for experimental study of pulmonary hypertension.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1999年第2期183-184,共2页
Chinese Journal of Experimental Surgery
关键词
左向右分流
肺动脉高压
动物模型
犬
Pulmonary hypertension ,Left-to-right shunt, Animal model, canine
