摘要
制各大鼠ＢＥＲＨ－２肝癌细胞和激活Ｂ细胞融合肿瘤疫苗并观察其免疫相关表型，为融合肿瘤疫苗应用人体提供依据。方法：应用ＰＥＧ在不同条件下，将肝癌细胞与激活Ｂ细胞融合。流式细胞仪鉴定融合细胞比例和检测ＭＨＣ－／Ⅱ、Ｂ７、ＩＣＡＭ－１、ＬＦＡ－１的水平。结果：５０％ＰＥＧ－１４００介导细胞融合６０秒获得融合细胞百分比最高。与融合前ＢＥＲＨ－２细胞相比，融合细胞获得了ＭＨＣ－Ⅱ、Ｂ７、ＩＣＡＭ－１、ＬＦＡ－１的表达，ＭＨＣ－Ｉ表达增加。结论：制备大鼠ＢＥＲＨ－２肝癌细胞和激活Ｂ细胞融合肿瘤疫苗的最佳条件为５０％ＰＥＧ－１４００融合６０秒。融合细胞不但表达肿瘤抗原，同时表达免疫原性相关分子。
Objective: To prepare cellular tumor vaccine by fusion of rat Beth - 2 hepatic tumor cellwith activated B lymphocytes and to observe immune related phenotype of the fused cells. Methods: Cellfusion was carried out in different conditions, in which PEG was used as the fusing agent. The identifica-tion of fused cells and the expression level of MHC - I/II, B7, ICAM - I and LFA - 1 were analysed byflow cytometry. Results: The highest cell fusion rate was achieved under the condition that 50% PFG -1400 was used as the fusing agent and fusing time lasted 60 seconds. Compared with parental BERH -2 cells, fusion cells not only expressed MHC -II, B7, ICAM - I and LFA - 1, but also expressedMHC - 1 to a higher level. Conclusion: The best fusing condition was that cells should be kept in 50%PEG - 1400 for 60s. Immunogenicity of the tumor cells can be enhanced by fusion with activated B lym-phocytes.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1999年第3期219-221,共3页
Chinese Journal of Clinical Oncology
基金
国家自然科学基金!39789010
国家杰出青年科学基金!39725028
上海市卫生局医学领先学科
