摘要
目的检测革兰阴性杆菌临床分离株的AmpC酶和AmpC耐药基因,探讨产AmpC酶菌株的耐药情况。方法用超声破碎法提取102株细菌的β-内酰胺酶粗提物,进行三维试验,提取细菌总DNA,PCR扩增ampC结构基因和ACT-1、CMY-G1、CMY-G2、DHA、FOX耐药基因,MIC法药物敏感试验分析菌株的耐药性。结果 102株革兰阴性杆菌中三维试验和PCR检测AmpC基因同时阳性的有11株,确认产AmpC酶菌株11株,并全部为鲍曼不动杆菌。三维试验和PCR基因检测AmpC酶的检出率比较,差别无统计学意义（χ~2=1.125,P〉0.05）。ACT-1、CMY-G2、FOX、DHA、CMY-G1的检出率分别为14.7%、12.7%、4.9%、1.0%和0。对产AmpC酶菌株敏感度最高的是丁胺卡那霉素（90.1%）,其次是亚胺培南（54.6%）。结论三维试验和PCR基因检测对AmpC酶的检出率无明显差别,但仍存在假阳性与假阴性。产AmpC酶菌株耐药情况严重,提示临床慎重用药。
Aim To detect AmpC β-lactamases and ampC gene of 102 gram-negative bacillus strains.Methods β-lactamases of the 102 gram-negative bacillus strains were extracted by ultrasonic crushing,and AmpC β-lactamases was examined by cefoxitin in three dimensional test.The total DNA of the 102 gram-negative bacillus strains were extracted,and AmpC gene was amplified by polymerase chain reaction（PCR）The drug resistance was analysed by MIC method.Results There 11 straina were poaitive for AmpC gene both by three-dimensional test and PCR detection.These 11 strains were confirmed to be AmpC-producing strains of Acinetobacter baumannii,without showing significant difference between the two methods（χ~2=1.125,P0.05）.The genotypes of AmpC β-lactamases were ACT-1（14.7%）,CMY-G2（12.7%）,FOX（4.9%）,DHA（1.0%）,CMY-G1（0）.Amikacin（90.1%）was the highest sensitive of the AmpC-producing strains,followed by imipenem（54.6%）.Conclusions There are no significant difference in the detection rate between three-dimensional test method and PCR method.The drug resistance of AmpC-producing strains is high and clinical application of drugs can only be used bsed on drug sensitivity tests.
出处
《中国热带医学》
CAS
2010年第9期1059-1061,共3页
China Tropical Medicine
关键词
革兰阴性杆菌
AMPC
三维试验
耐药性
Gram-negative bacilli
AmpC
Three-dimensional test
Drug resistance
