摘要
从海南岛采集了桶形芋螺标本１０ｋｇ。比较了“超低温脆化法”，“匀浆法”，“剥离法”的取毒效果。经凝胶过滤色谱分离后，共得粗毒液 ５０４ｍｇ，其中，Ｐ１，２１０ｍｇ，分子量 Ｍｗ＞５０００；Ｐ２，１８４ｍｇ，Ｍｗ＝１０００～５０００；Ｐ３，５０ｍｇ，Ｍｗ＜１０００。用高效液相色谱（ＨＰＬＣ）和高效毛细管电泳（ＨＰＣＥ）对 Ｐ２，Ｐ３进行了比较研究。毒液经 ＨＰＬＣ分离后得到数十个成分，各成分多数在２０ｍｇ以内。药理实验表明，它们具有一系列中枢神经活性（ＣＮＳ）。
kg of Conus betulinus were collected from South China Sea. Different extraction methods were compared for their yields of venoms. 504mg of the crude venom, which was composed of P1 (210mg, Mw> 5000), P2 (184mg, Mw=1000～5000)and P3 (50mg, Mw <1000),was subjected to gel filtration,HPLC and HPCE for component identification. Tens of components purified from HPLC showed a series of CNS activities on mice(i. c. ).
出处
《中国海洋药物》
CAS
CSCD
1999年第1期1-6,共6页
Chinese Journal of Marine Drugs
