期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

CENP-E变异体在不同肿瘤细胞株及癌组织中的表达差异 被引量：2

Difference in Expressions of CENP-E Variant in Various Tumor Cell Strains and Carcinoma Tissues
原文传递
导出
摘要 目的研究CENP-E变异体(CENP-EⅠ)在不同肿瘤细胞株及癌组织和相应的癌旁组织中的表达情况。方法应用巢式RT-PCR检测14个细胞株和17份癌组织及相应癌旁组织标本中CENP-EⅠmRNA(缺失第38个外显子)的表达情况,并比较变异体和野生型CENP-E(CENP-EWT) mRNA的表达差异;应用巢式PCR检测上述标本中CENP-E在DNA水平上的变异;并通过细胞增殖试验、细胞周期检测和染色体核型鉴定,分析CENP-EⅠ与细胞恶性程度的相关性。结果 RT-PCR结果显示,实验样本中CENP-E mRNA均有CENP-EWT和CENP-EⅠ两种形式同时存在于一种组织或细胞中,且在正常细胞株和癌旁组织中CENP-EWT表达量较高,在肿瘤细胞株和癌组织中CENP-EⅠ表达量较高,且差异有统计学意义(P<0.05);样本中CENP-E在DNA水平上不存在第38个外显子缺失,且细胞间CENP-E表达量的差异无统计学意义(P>0.05);CENP-EⅠ与细胞的恶性程度有一定的相关性。结论同一细胞内存在2种或2种以上形式的CENP-E mRNA,CENP-E变异体可能与肿瘤的发生有关。 Objective To investigate the expressions of CENP-EⅠ,a CENP-E variant,in various tumor cell strains as well as peri-cancerous and carcinoma tissues.Methods The expressions of mRNA of CENP-EⅠ,a CENP-E variant with deletion of the 38th exon,in 14 cell strains as well as 17 carcinoma tissue specimens and their corresponding peri-cancerous tissue specimens were determined by nested RT-PCR,and compared with those of wild type CENP-E（CENP-EWT）.The variations of CENP-E at DNA level in the above-mentioned specimens were determined by nested PCR.The relationship of CENP-EⅠ to the malignant degree of cells were analyzed by cell proliferation test,determination of cell cycle and identification of chromosome karyotype.Results RT-PCR proved that both CENP-EWT and CENP-EⅠexisted in the same tissue or cell strain.CENP-EWT was highly expressed in normal cell strains and peri-cancerous tissues,while CENP-EⅠ in tumor cell strains and carcinoma tissues,which showed significant difference（P 0.05）.No deletion of the 38th exon at DNA level was observed in the specimens,and the expression levels of CENP-E in various cells showed no significant difference.However,CENP-EⅠ showed a certain relationship to the malignant degree of cells.Conclusion The CENP-E mRNA in not less than two forms may exist in the same cell strain,and CENP-E variant may be related to tumorigenesis.
作者 闫琛 凌康 刘子杰 翁亚光
机构地区 重庆医科大学医学检验系临床检验诊断学教育部重点实验室重庆市重点实验室 重庆医科大学第一临床学院外科
出处 《中国生物制品学杂志》 CAS CSCD 2010年第7期678-682,691,共6页 Chinese Journal of Biologicals
基金 教育部博士点基金资助项目(20060631012)
关键词 纺锤体检查点 CENP-E 变异体 肿瘤 Spindle assemble checkpoint CENP-E Variant Tumor
分类号 R730.231 [医药卫生—肿瘤]
  • 相关文献

参考文献13

  • 1Yuen KW, Desai A. The wages of CIN [J]. J Cell Biol, 2008,180 (4): 661-663.
  • 2Yen TJ, Li G, Schaar BT, et al. CENP-E is a putative kinetochore motor that accumulates just before mitosis [J]. Nature, 1992,359 (6395): 536-539.
  • 3Tanudji M, Shoemaker J, L'italien L, et al. Gene silencing of CENP-E by small interfering RNA in HeLa cells leads to missegregation of chromosomes after a mitotic delay [J]. Mol Biol Cell,2004, 15 (8): 3771-3781.
  • 4Malmanche N, Maia A, Sunkel CE. The spindle assembly checkpoint: preventing chromosome mis-segregation during mitosis and meiosis [J]. FEBS Lett, 2006, 580 (12): 2888-2895.
  • 5Yardimci H, van Duffelen M, Mao Y, et al. The mitotic kinesin CENP-E is a processive transport motor[J]. Proc Natl Acad Sci USA, 2008, 105 (16): 6016-6021.
  • 6Mao Y, Desai A, Cleveland DW. Microtubule capture by CENP-E silences BubRl-dependent mitotic checkpoint signaling [J]. J Cell Biol, 2005, 170 (6): 873-880.
  • 7Kim Y, Heuser JE, Waterman CM, et al.CENP-E combines a slow,processive motor and a flexible coiled coil to produce an essential motile kinetochore tether [J]. J Cell Biol, 2008, 181 (3): 411-419.
  • 8Abrieu A, Kahana JA, Wood KW, et all. CENP-E as an essential component of the mitotic checkpoint in vitro [J]. Cell, 2000, 102 (6): 817-826.
  • 9Weaver BA, Silk AD, Montagna C, et al.Aneuploidy acts both oncogenically and as a tumor suppressor [J]. Cancer Cell, 2007, 11 (1): 25-36.
  • 10Grimm D, Kay MA. RNAi and gene therapy: a mutual attraction [J]. Hematology Am Soc Hematol Educ Program,2007 (1): 473- 481.

二级参考文献18

  • 1May K M, Hardwick K G. The spindle checkpoint. J Cell Sci, 2006,119( Pt 20) :4139 - 4142
  • 2Kops G J. The kinetochore and spindle checkpoint in mammals. Front Biosci, 2008,13:3606 - 3620
  • 3Taylor S S, Scott M I, Holland A J. The spindle checkpoint: a quality control mechanism which ensures accurate chromosome segregation. Chromosome Res, 2004,12 ( 6 ) :599 - 616
  • 4Vigneron S, Prieto S, Bemis C, et al. Kinetochore localization of spindle checkpoint proteins: who controls whom?. Mol Biol Cell, 2004,15(10) :4584 -4596
  • 5Garcia-Saez I, Yen T, Wade R H, et al. Crystal structure of the motor domain of the human kinetochore protein CENP-E. J Mol Biol, 2004,340(5) :1107 - 1116
  • 6Liu D, Zhang N, Du J, et al. Interaction of Skpl with CENP-E at the midbody is essential for cytokinesis. Biochem Biophys Res Commun, 2006,345( 1 ) :394 -402
  • 7Brown K D, Wood K W, Cleveland D W. The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A. J Cell Sci, 1996, 109 ( Pt 5) :961-969
  • 8Karpova T S, Baunmnn C T, He L, et al. Fluorescence resonance energy transfer from cyan to yellow fluorescent protein detected by acceptor photobleaching using corffocal microscopy and a single laser. J Microsc, 2003,209(Pt 1 ) :56 -70
  • 9Lleres D, Swift S, Lamond A I. Detecting protein-protein interactions in vivo with FRET using muhiphoton fluorescence lifetime imaging microscopy (FLIM). Curt Protoc Cytom, 2007 ,Chapter 12 :Unitl2.10
  • 10Stucke V M, Baumann C, Nigg E A. Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mpsl. Chromosoma, 2004,1 1 3 ( 1 ) : 1 - 15

共引文献3

同被引文献30

  • 1Cahill DP,Lengauer C,Yu J,et al.Mutations of mitotic checkpointgenes in human cancers[J].Nature,1998,392(6673):300-303.
  • 2Qi W,Yu H.The spindle checkpoint and chromosomal stability[J].Genome Dis,2006,1:116-130.
  • 3Semizarov D,Frost I,Sarthy A,et al.Specificity of short inter-fering RNA determined through gene expression signatures[J].Proc Natl Acad Sci USA,2003,100(11):6347-6352.
  • 4Neumann E,Garcia-Saez I,DeBonis S,et al.Human kinetochore-associated kinesin CENP-E visualized at 17 A resolution bound tomicrotubules[J].J Mol Biol,2006,362(2):203-211.
  • 5Kim Y,Heuser JE,Waterman CM,et al.CENP-E combines aslow,processive motor and a flexible coiled coil to produce an es-sential motile kinetochore tether[J].J Cell Biol,2008,181(3):411-419.
  • 6Yardimci H,van Duffelen M,Mao Y,et al.The mitotic kinesinCENP-E is a processive transport motor[J].Proc Natl Acad SciUSA,2008,105(16):6016-6021.
  • 7Wierinckx A,Auger C,Devauchelle P,et al.A diagnosticmarker set for invasion,proliferation,and aggressiveness of pro-lactin pituitary tumors[J].Endocr Relat Cancer,2007,14(3):887-900.
  • 8Weaver BA,Silk AD,Montagna C,et al.Aneuploidy acts bothoncogenically and as a tumor suppressor[J].Cancer Cell,2007,11(1):25-36.
  • 9Liu ZJ,Ling K,Wu X,et al.Reduced expression of cenp-e inhuman hepatocellular carcinoma[J].J Exp Clin Cancer Res,2009,28(1):156-163.
  • 10Janssen A,Kops GJ,Medema RH.Elevating the frquency ofchromosome mis-segregation as a strategy to kill tumor cells[J].Proc Natl Acad Sci US,2009,106(45):19108-19113.

引证文献2

中国生物制品学杂志
2010年 第7期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部