摘要
以葡萄栽培品种红宝石无核和砧木SO4的单芽茎段为材料,研究葡萄的初代培养与增殖培养。结果表明,升汞的最佳消毒时间为12min、13min;红宝石无核最适初代培养基为1/2MS+6-BA1.0mg/L+NAA0.1mg/L,砧木SO4的最适培养基为1/2MS+6-BA1.5mg/L+NAA0.1mg/L;红宝石无核继代增殖的最适培养基为MS+6-BA0.8mg/L+IBA0.2mg/L,砧木SO4为MS+6-BA1.0mg/L+IBA0.2mg/L。两品种最佳生根培养基为1/2MS+IBA0.4mg/L,红宝石无核生根率达98%,而SO4则达100%。
The commonly cultivated Vitis cv. Ruby Seedless and rootstock SO4 were used as experiment materials to study the multiplication of single bud stem segment cultured in vitro. The results showed that the sterilization of 0.1% HgCI for 12min and 13min could have the best effect. The best initial culture medium of Ruby Seedless was 1/2MS＋6-BA1.0mg/L＋NAA0.1mg/L, and that best for rootstock SO4 was 1/2MS＋6-BA 1.5mg/L ＋NAA 0.1 mg/L. The best subculture medium for Ruby Seedless and SO4 were MS＋6-BA0.Smg/L＋IBA0.2mg/L and MS＋6-BA1.0mg/L＋IBA0.2mg/L. Rooting medium （1/2MS＋IBA0.4mg/L） could maximize the rooting rate of Ruby Seedless and SO4 to be 98% and 100%.
出处
《中外葡萄与葡萄酒》
2010年第4期28-30,33,共4页
Sino-Overseas Grapevine & Wine
基金
现代农业产业技术体系建设专项资金资助
关键词
葡萄茎段
组织培养
增殖培养
grapevine stem
tissue culture
multiplication
