摘要
以58头草原红牛为试验材料,采用PCR-SSCP技术对胰岛素样生长因子Ⅰ受体(IGF-ⅠR)基因3′UTR序列多态性进行了检测。结果表明,在IGF-ⅠR基因3′UTR中发现PCR-SSCP的多态位点,有2种等位基因和3种基因型。对所得基因型与生长和胴体性状的相关性分析结果发现,在3′UTR中AB型的胴体产肉率、肉骨比显著高于AA、BB型(P<0.05);AA型的脂肪覆盖率显著高于BB型(P<0.05),与AB型差异不显著(P>0.05)。
The 3′UTR polymorphism of IGF-ⅠR gene was detected by PCR-SSCP in 58 red steppes.The results showed that two allelomorphic gene,three genotype of IGF-ⅠR gene were detected.The associativity was analyzed between the polymorphism of slaughter and carcass traits.The results indicated that procured meat percentage and the ratio of meat in bone for AB genotype was higher than AA and BB genotypes(P0.05),the rate of fat coverage for AA genotype was higher than BB genotype(P0.05),and was no significant difference with AB genotype(P0.05).
出处
《中国畜牧兽医》
CAS
北大核心
2010年第7期103-106,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家"863"计划项目(2008AA101010)
国家支撑计划项目(2007BAD55B01)
关键词
草原红牛
IGF-ⅠR基因
3′UTR
多态性分析
red steppes
insulin-like growth factor Ⅰ receptor(IGF-ⅠR)
3′UTR
single nucleotide polymorphism