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牦牛PIT-1基因第5、6外显子部分序列的克隆测序 被引量:2

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摘要 利用PCR方法扩增了麦洼牦牛PIT-1基因第5、6外显子的部分序列,扩增片段长度为1 285 bp,克隆到PND-18载体中进行测序,结果牦牛与普通牛的PIT-1基因有97%的同源性,共有28处不同,其中在内含子5的347处有18个碱基的缺失,565处有4个碱基的插入;外显子6有一个碱基突变,并导致氨基酸由普通牛的Thr变为牦牛的Arg。对61头麦洼牦牛和30头九龙牦牛PIT-1基因部分序列进行PCR-RFLP分析,均未检测到多态性。
出处 《江苏农业科学》 CSCD 北大核心 2006年第4期91-93,共3页 Jiangsu Agricultural Sciences
基金 国家自然科学基金(编号:39870607)
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参考文献11

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