摘要
应用超速离心技术对中国明对虾(Fenneropenaeus chinensis)血浆样品进行处理,比较去除和未去除高丰度蛋白血浆的双向凝胶电泳(2-DE)图谱。结果表明:对虾血浆高丰度蛋白经去除后,血浆样品中的低丰度蛋白得到较好的分离和浓缩。未处理样品的2-DE图谱分离的蛋白质170个,而去除高丰度蛋白后血浆2-DE图谱上分辨率增高,分离出蛋白质点520个,一些低丰度蛋白质被有效分离出来。研究证实,超速离心技术可有效去除对虾血浆高丰度蛋白(血蓝蛋白),提高低丰度蛋白的检出敏感性,为对虾等甲壳动物的血浆蛋白质组学研究提供了基础保障。
To establish the method for removing high-abundance proteins in shrimp plasma proteomics analysis, depletion of plasma high-abundance proteins,such as hemocyanins,was practiced by ultracentrifugation. The efficiency of the method was assessed by 2-DE. The ultracentrifuge method for removing plasma high-abundance proteins was established to remove most hemocyanins. The proteins spots of 2-DE map were more clear and some low-abundance proteins were visualization after ultracentrifugation. The established methods for high-abundance depletion can be effectively applied in shrimp and other crustaceans plasma proteomic study.
出处
《中国水产科学》
CAS
CSCD
北大核心
2010年第4期695-700,共6页
Journal of Fishery Sciences of China
基金
国家自然科学基金项目(30600458)
关键词
中国明对虾
血浆
蛋白质组
双向电泳
高丰度蛋白去除
血蓝蛋白
超速离心
Fenneropenaeus chinensis
plasma
proteomics
2-DE
high abundance proteins depletion
hemocyanin
ultracentrifugation
