摘要
[目的]研究二色补血草黄酮的抗氧化功能及其最佳提取工艺。[方法]采用超声-微波协同法及L9(34)正交试验设计优化二色补血草黄酮的提取工艺,利用羟自由基抑制和油脂抗氧化试验判断二色补血草中黄酮的抗氧化功能。[结果]二色补血草中黄酮的最佳提取工艺为:乙醇浓度40%,料液比1∶50,温度50℃,提取时间20 min;此条件下提取量为50.97 mg/g。二色补血草黄酮类提取物对羟自由基.OH有明显的清除作用,并可抑制油脂的酸败,且作用效果随着浓度的增加而增强。[结论]该研究为二色补血草的综合开发和在保健食品及医药工业中的应用提供理论依据。
[Objective] Extraction process of flavonoids in Limonium bicolor and its antioxidation function were studied.[Method] Ultrasonic-microwave synergistic technique and orthogonal design were applied in the extraction process.The antioxidant capacity of flavonoids in Limonium bicolor was estimated with the test of hydroxyl radical inhibition and lipid antioxidation.[Result] The optimal extraction paramenters were as follow： ethanol concentration 40%,solid-liquid ratio 1∶50,temperature 50 ℃,extraction time 20 min.Under the optimized conditions,the extraction yield of flavonoids in Limonium bicolor was 50.97 mg/g.The test of antioxidant capacity showed that radical ·OH was eliminated significantly,lipid rancidification was inhibited,and the role of these effect increased with the increase of flavonoids concentration.[Conclusion] The study provided theoretical basis for the comprehensive development and utilization of Limonium bicolor in the health food and pharmaceutical industries.
出处
《安徽农业科学》
CAS
北大核心
2010年第16期8665-8666,共2页
Journal of Anhui Agricultural Sciences
