摘要
目的初步探讨骨碎补水提液、醇提液以及骨碎补的有效成分柚皮甙对人骨髓间充质干细胞(hu-manbonemarrowmesenchymalstemcells,hMSCs)增殖、分化的影响。方法将骨碎补水提液、醇提液和骨碎补的有效成分柚皮甙与hMSCs共同体外培养,倒置显微镜观察描述细胞生长情况、CCK-8法检测每组药液对细胞的毒性和增殖作用、碱性磷酸酶(ALP)活性测定,图文报告分析系统检测VonKossa染色的钙结节,统计学处理所得数据。结果与空白对照组比较,1mg/L骨碎补水提液、50μg/L骨碎补醇提液与50μg/L柚皮甙能促进hMSCs增加(P<0.05),50μg/L骨碎补醇提液与50μg/L柚皮甙能促进其向成骨细胞分化(P<0.05)。结论骨碎补水提液、醇提液与柚皮甙对hMSCs有保护作用并能分别促进其增殖、分化。
[Objective] To investigate the state and differentiation of human bone marrow mesenchymal stem cells into osteoblasts induced by Naringin and the other ingredients of Drynaria Rhizome such as ingredients of Drynaria Rhizome extracted by water or alcohol, [Methods] hMSCs were harvested by puncturing iliac of volunteers . The hMSCs were induced by several ingredients of Drynaria Rhizone.The state of hMSCs were detected by phasecontrast microscope and Cell Counting Kit-8 （CCK-8） Alkaline phosphotase （ALP） and Von Kossa. [Results] 1 mg/ L ingredients of Drynaria Rhizone extracted by water, 50 ug/L ingredients of Drynaria Rhizone extracted by alcohol and 51 ug/L Naringin can accelerate the proliferation of hMSCs （P 〈0.05）： 50 ug/L ingredients of Drynaria Rhizone extracted by alcohol and 50 ug/L Naringin can acted as osteoblast inducers. [Conclusion] Ingredients of Drynaria Rhizone extracted by water or alcohol and Naringin can accelerate the proliferation and induce hMSCs into osteoblasts.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第16期2426-2429,共4页
China Journal of Modern Medicine
基金
湖南省卫生厅中医药科研基金资助课题(No:204043)
